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Titolo:
HUMAN GAMMA-X SATELLITE DNA - AN X-CHROMOSOME SPECIFIC CENTROMERIC DNA-SEQUENCE
Autore:
LEE C; LI X; JABS EW; COURT D; LIN CC;
Indirizzi:
UNIV ALBERTA,DEPT LAB MED & PATHOL EDMONTON AB T6G 2B7 CANADA UNIV ALBERTA,DEPT LAB MED & PATHOL EDMONTON AB T6G 2B7 CANADA UNIV ALBERTA HOSP EDMONTON AB T6G 2B7 CANADA JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,CTR GENET MED BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT MED BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT SURG BALTIMORE MD 21205
Titolo Testata:
Chromosoma
fascicolo: 2, volume: 104, anno: 1995,
pagine: 103 - 112
SICI:
0009-5915(1995)104:2<103:HGSD-A>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; HUMAN ACROCENTRIC CHROMOSOMES; ALPHA-SATELLITE; REPETITIVE DNA; CENP-B; PERICENTROMERIC REGIONS; HIGH-FREQUENCY; ORGANIZATION; LOCALIZATION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
C. Lee et al., "HUMAN GAMMA-X SATELLITE DNA - AN X-CHROMOSOME SPECIFIC CENTROMERIC DNA-SEQUENCE", Chromosoma, 104(2), 1995, pp. 103-112

Abstract

The cosmid clone, CX16-2D12, was previously localized to the centromeric region of the human X chromosome and shown to lack human X-specific a satellite DNA. A 1.2 kb EcoRI fragment was subcloned from the CX16-2D12 cosmid and was named 2D12/E2. DNA sequencing revealed that this 1,205 bp fragment consisted of approximately five tandemly repeated DNA monomers of 220 bp. DNA sequence homology between the monomers of 2D12/E2 ranged from 72.8% to 78.6%. Interestingly, DNA sequence analysis of the 2D12/E2 clone displayed a change in monomer unit orientation between nucleotide positions 585-586 from a ''tail-to-head'' arrangement to a ''head-to-tail'' configuration. This may reflect the existenceof at least one inversion within this repetitive DNA array in the centromeric region of the human X chromosome. The DNA consensus sequence derived from a compilation of these 220 bp monomers had approximately 62% DNA sequence similarity to the previously determined gamma 8 satellite DNA consensus sequence. Comparison of the 2D12/E2 and gamma 8 consensus sequences revealed a 20 bp DNA sequence that was well conservedin both DNA consensus sequences. Slot-blot analysis revealed that this repetitive DNA sequence comprises approximately 0.015% of the human genome, similar to that found with gamma 8 satellite DNA. These observations suggest that this satellite DNA clone is derived from a subfamily of gamma satellite DNA and is thus designated gamma X satellite DNA, When genomic DNA from six unrelated males and two unrelated females was cut with SstI or HpaI and separated by pulsed-field gel electrophoresis, no restriction fragment length polymorphisms were observed for either gamma X (2D12/E2) or gamma 8 (50E4) probes. Fluorescence in situ hybridization localized the 2D12/E2 clone to the lateral sides of the primary constriction specifically on the human X chromosome.

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Documento generato il 25/09/20 alle ore 04:48:06