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Titolo:
CHANGES IN THE SIALYLGLYCOCONJUGATE DISTRIBUTION ON THE HUMAN SPERM SURFACE DURING IN-VITRO CAPACITATION - PARTIAL-PURIFICATION OF A 20 KDASIALYLGLYCOPROTEIN OF CAPACITATED SPERMATOZOA
Autore:
FOCARELLI R; GIUFFRIDA A; ROSATI F;
Indirizzi:
UNIV SIENA,DEPT EVOLUT BIOL,VIA MATTIOLI 4 I-53100 SIENA ITALY UNIV SIENA,DEPT EVOLUT BIOL I-53100 SIENA ITALY
Titolo Testata:
Human reproduction
fascicolo: 10, volume: 10, anno: 1995,
pagine: 2755 - 2759
SICI:
0268-1161(1995)10:10<2755:CITSDO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; INVITRO CAPACITATION; SIALIC-ACID; LECTIN; MEMBRANE; BINDING; ASSAY; NEURAMINIDASE; RECOGNIZES; CELLS;
Keywords:
CAPACITATION; GLYCOCONJUGATES; HUMAN SPERMATOZOA; LECTINS; SIALIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
R. Focarelli et al., "CHANGES IN THE SIALYLGLYCOCONJUGATE DISTRIBUTION ON THE HUMAN SPERM SURFACE DURING IN-VITRO CAPACITATION - PARTIAL-PURIFICATION OF A 20 KDASIALYLGLYCOPROTEIN OF CAPACITATED SPERMATOZOA", Human reproduction, 10(10), 1995, pp. 2755-2759

Abstract

Changes in the distribution of sialylglycoconjugates on the surface of uncapacitated and in-vitro capacitated human spermatozoa were studied by means of two sialic acid specific lectins (Maackia amurensis agglutinin and Sambucus nigra agglutinin). On the uncapacitated sperm surface, sialylglycoconjugates were found to be localized from the post-acrosomal region of the sperm head to the tail middle piece, whereas after in-vitro capacitation these molecules were only found in a small area of the post-acrosomal region, The surface of capacitated human spermatozoa was also investigated by specifically radiolabelling its terminal sialic acid residues, A 20 kDa glycoprotein, which was partially purified by anion-exchange chromatography, was the main component of the sialylglycoconjugate pattern after in-vitro capacitation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:59:06