Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
QUANTIFICATION OF THE EASE OF REMOVAL OF BACTERIA FROM SURFACES
Autore:
EGINTON PJ; GIBSON H; HOLAH J; HANDLEY PS; GILBERT P;
Indirizzi:
UNIV MANCHESTER,DEPT PHARM,OXFORD RD MANCHESTER M13 9PL LANCS ENGLAND UNIV MANCHESTER,DEPT PHARM MANCHESTER M13 9PL LANCS ENGLAND CAMPDEN FOOD & DRINK RES ASSOC CHIPPING CAMPDEN GL55 6LD GLOS ENGLAND UNIV MANCHESTER,SCH BIOL SCI MANCHESTER M13 9PL LANCS ENGLAND
Titolo Testata:
Journal of industrial microbiology
fascicolo: 4, volume: 15, anno: 1995,
pagine: 305 - 310
SICI:
0169-4146(1995)15:4<305:QOTEOR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-AERUGINOSA BIOFILM; ESCHERICHIA-COLI; GROWTH-RATE; RESISTANCE; DISPERSAL; MECHANISM; CYCLE;
Keywords:
BIOFILMS; SURFACE-ATTACHMENT; SURFACE-DETACHMENT; HYGIENE; CONTAMINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Eginton et al., "QUANTIFICATION OF THE EASE OF REMOVAL OF BACTERIA FROM SURFACES", Journal of industrial microbiology, 15(4), 1995, pp. 305-310

Abstract

This paper describes a technique which reproducibly quantifies the ease of removal of microorganisms from surfaces. Tiles (22 mm x 22 mm) of various materials were colonised with Staphylococcus epidermidis NCTC 11047, Escherichia coil K12 HB101 or Pseudomonas aeruginosa PaWH, bysubmersion, for various times (2 min-48 h), in inoculated Tryptone Soya broth (37 degrees C). Colonised tiles were blotted onto a Tryptone Soya agar plate for 1 min and the process was repeated through a succession of agar plates. The final plate contained tetrazolium salts (0.05% w/v) and was incubated in situ with the tile. Tetrazolium plates indicated that very few organisms remained on the tiles after 15 successive blots. In all instances, the number of recovered colonies per plate decreased exponentially with plate succession number, according to the relationship, CFU = A.10-(kN), where CFU is the number of colonies transferred, k is the removal exponent, A is the intercept and N is the plate succession number. Removal exponents differed significantly between organisms (P > 0.95), depended on the nature of the test surface, and decreased as the inital attachment and colonisation time was increased from 2 min-48 h. Intercept values (A) but not the gradients were dependent upon the initial numbers of bacteria in suspension. These data indicate that the gradients derived from counting recoverable viable cells from successive blots of test tiles onto agar is a measure of the strength of attachment of the organisms to the surface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:29:49