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Titolo:
TARGETED MUTAGENESIS OF THE TRANSCRIPTION FACTOR GATA-4 GENE IN MOUSEEMBRYONIC STEM-CELLS DISRUPTS VISCERAL ENDODERM DIFFERENTIATION IN-VITRO
Autore:
SOUDAIS C; BIELINSKA M; HEIKINHEIMO M; MACARTHUR CA; NARITA N; SAFFITZ JE; SIMON MC; LEIDEN JM; WILSON DB;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT PEDIAT,BOX 8116,1 CHILDRENS PL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT PEDIAT ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT PATHOL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT MOLEC BIOL & PHARMACOL ST LOUIS MO 63110 UNIV CHICAGO,SCH MED,DEPT MED CHICAGO IL 00000 UNIV CHICAGO,SCH MED,DEPT MOLEC GENET & CELL BIOL CHICAGO IL 00000 UNIV CHICAGO,SCH MED,DEPT PATHOL CHICAGO IL 00000 UNIV CHICAGO,SCH MED,HOWARD HUGHES MED INST CHICAGO IL 00000 UNIV HELSINKI,CHILDRENS HOSP HELSINKI FINLAND
Titolo Testata:
Development
fascicolo: 11, volume: 121, anno: 1995,
pagine: 3877 - 3888
SICI:
0950-1991(1995)121:11<3877:TMOTTF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTO-ONCOGENE INT-2; YOLK-SAC; CARCINOMA-CELLS; EXPRESSION; BINDING; BODIES; HEART; VASCULOGENESIS; ANGIOGENESIS; TISSUES;
Keywords:
KNOCKOUT; YOLK SAC; PRIMITIVE ENDODERM; MUTAGENESIS; GATA-4; MOUSE; VISCERAL ENDODERM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
C. Soudais et al., "TARGETED MUTAGENESIS OF THE TRANSCRIPTION FACTOR GATA-4 GENE IN MOUSEEMBRYONIC STEM-CELLS DISRUPTS VISCERAL ENDODERM DIFFERENTIATION IN-VITRO", Development, 121(11), 1995, pp. 3877-3888

Abstract

Transcription factor GATA-4 belongs to a family of zinc finger proteins involved in lineage determination, GATA-4 is first expressed in yolk sac endoderm of the developing mouse and later in cardiac tissue, gut epithelium and gonads. To delineate the role of this transcription factor in differentiation and early development, we studied embryoid bodies derived from mouse embryonic stem (ES) cells in which both copiesof the Gata4 gene were disrupted. Light and electron microscopy demonstrated that embryoid bodies formed from wild-type and heterozygous deficient ES cells were covered with a layer of visceral yolk sac endoderm, whereas no yolk sac endoderm was evident on the surface of the homozygous deficient embryoid bodies. Independently selected homozygous deficient cell lines displayed this distinctive phenotype, suggesting that it was not an artifact of clonal variation. Biochemical markers ofvisceral endoderm formation, such as alpha-feto-protein, hepatocyte nuclear factor-4 and binding sites for Dolichos biflorus agglutinin, were absent from the homozygous deficient embryoid bodies. Examination of other differentiation markers in the mutant embryoid bodies, studiesof ES cell-derived teratocarcinomas and chimeric mouse analysis demonstrated that GATA-4-deficient ES cells have the capacity to differentiate along other lineages. We conclude that, under in vitro conditions,disruption of the Gata4 gene results in a specific block in visceral endoderm formation. These homozygous deficient cells should yield insights into the regulation of yolk sac endoderm development and the factors expressed by visceral endoderm that influence differentiation of adjoining ectoderm/mesoderm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 16:06:24