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Titolo:
BOVINE LATENT TRANSFORMING GROWTH-FACTOR BETA-1-BINDING PROTEIN-2 - MOLECULAR-CLONING, IDENTIFICATION OF TISSUE ISOFORMS, AND IMMUNOLOCALIZATION TO ELASTIN-ASSOCIATED MICROFIBRILS
Autore:
GIBSON MA; HATZINIKOLAS G; DAVIS EC; BAKER E; SUTHERLAND GR; MECHAM RP;
Indirizzi:
UNIV ADELAIDE,DEPT PATHOL ADELAIDE SA 5005 AUSTRALIA WOMENS & CHILDRENS HOSP,DEPT CYTOGENET & MOLEC GENET ADELAIDE SA 5006AUSTRALIA WASHINGTON UNIV,MED CTR,DEPT CELL BIOL ST LOUIS MO 63110
Titolo Testata:
Molecular and cellular biology
fascicolo: 12, volume: 15, anno: 1995,
pagine: 6932 - 6942
SICI:
0270-7306(1995)15:12<6932:BLTGBP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARFAN-SYNDROME; MONOCLONAL-ANTIBODY; GLYCOPROTEIN MAGP; FIBRILLIN; COMPONENT; GENE; ORGANIZATION; PURIFICATION; TGF-BETA-1; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Gibson et al., "BOVINE LATENT TRANSFORMING GROWTH-FACTOR BETA-1-BINDING PROTEIN-2 - MOLECULAR-CLONING, IDENTIFICATION OF TISSUE ISOFORMS, AND IMMUNOLOCALIZATION TO ELASTIN-ASSOCIATED MICROFIBRILS", Molecular and cellular biology, 15(12), 1995, pp. 6932-6942

Abstract

Monoclonal antibodies to fibrillin 1 (MP340), a component of elastin-associated microfibrils, were used to screen cDNA libraries made from bovine nuchal ligament mRNA. One of the selected clones (cL9; 1.2 kb) hybridized on Northern (RNA) blotting with nuchal ligament mRNA to twoabundant mRNAs of 9.0 and 7.5 kb, which were clearly distinct from fibrillin mRNA (10 kb). Further library screening and later reverse transcription PCR by the rapid amplification of cDNA ends (RACE) techniqueresulted in the isolation of additional overlapping cDNAs corresponding to about 6.7 kb of the mRNA. The encoded protein exhibited sequencesimilarity of around 80% with a recently identified human protein named latent transforming growth factor beta 1 (TGF-beta 1)-binding protein 2 (LTBP-2), indicating that the new protein was bovine LTBP-2. Thiswas confirmed by the specific localization of bovine LTBP-2 cDNA probes to human chromosome 14q24.3, which is the locus of the human LTBP-2gene. The domain structure of bovine LTBP-2 is very similar to that of the human LTBP-2, containing 20 examples of 6-cysteine epidermal growth factor-like repeats, 16 of which have the consensus sequence for calcium binding, together with 4 examples of 8-cysteine motifs characteristic of fibrillins and LTBP-1. A il-cysteine sequence which is unique to bovine LTBP-2 and which has similarity to the 8-cysteine motifs was also present. Antibodies raised to two unique bovine LTBP-2 peptides specifically localized in tissue sections to the elastin-associated microfibrils, indicating that LTBP-2 is closely associated with-these structures. Immunoblotting experiments identified putative LTBP-2 isoforms as a 260-kDa species released into the medium by cultured elastictissue cells and as larger 290- and 310-kDa species in tissue extracts. A major proportion of tissue-derived LTBP-2 required treatment with6 M guanidine for solubilization, indicating that the protein was strongly bound to the microfibrils. Most of the guanidine-solubilized LTBP-2 appeared to be monomeric, indicating that it was not involved in disulfide-bonded aggregation either with itself or with latent TGF-beta. Additional LTBP-2 was resistant to solubilization with 6 M guanidinebut was readily extracted with a-reductive saline solution. This treatment is relatively specific for solubilization of microfibrillar constituents including fibrillin 1 and microfibril-associated glycoprotein. Therefore, it can be inferred that some LTBP-2 is bound covalently to the microfibrils by reducible disulfide linkages. The evidence suggests that LTBP-2 has a direct role in elastic fiber structure and assembly which may be independent of its growth factor-binding properties. Thus, LTBP-2 appears to share functional characteristics with both LTBP-1 and fibrillins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:04:19