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Titolo:
KINETOPLAST MAXICIRCLE DNA-REPLICATION IN CRITHIDIA-FASCICULATA AND TRYPANOSOMA-BRUCEI
Autore:
CARPENTER LR; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,725 N WOLFE ST BALTIMORE MD21205 JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205
Titolo Testata:
Molecular and cellular biology
fascicolo: 12, volume: 15, anno: 1995,
pagine: 6794 - 6803
SICI:
0270-7306(1995)15:12<6794:KMDICA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; MITOCHONDRIAL-DNA; VARIABLE REGION; SACCHAROMYCES-CEREVISIAE; CELL-CYCLE; MINICIRCLES; PURIFICATION; ORGANIZATION; TRANSCRIPTION; ENDONUCLEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
L.R. Carpenter e P.T. Englund, "KINETOPLAST MAXICIRCLE DNA-REPLICATION IN CRITHIDIA-FASCICULATA AND TRYPANOSOMA-BRUCEI", Molecular and cellular biology, 15(12), 1995, pp. 6794-6803

Abstract

Kinetoplast DNA, the mitochondrial DNA of trypanosomatids, is composed of several thousand minicircles and a few dozen maxicircles, all of which are topologically interlocked in a giant network, We have studied the replication of maxicircle DNA, using electron microscopy to analyze replication intermediates from both Crithidia fasciculata and Trypanosoma brucei, Replication intermediates were stabilized against branch migration by introducing DNA interstrand cross-links in vivo with 4,5',8-trimethylpsoralen and UV radiation. Electron microscopy of individual maxicircles resulting from a topoisomerase II decatenation of kinetoplast DNA networks revealed intact maxicircle theta structures, Analysis of maxicircle DNA linearized by restriction enzyme cleavage revealed branched replication intermediates derived from theta structures. Measurements of the linearized branched molecules in both parasites indicate that replication initiates in the variable region (a noncoding segment characterized by repetitive sequences) and proceeds unidirectionally, clockwise on the standard map.

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Documento generato il 03/12/20 alle ore 21:18:38