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Titolo:
THE TEMPORAL RELATIONSHIPS OF SYNTHESIS AND PHOSPHORYLATION IN STRESSPROTEINS 70 AND 90 IN AGED CALORIC RESTRICTED RATS EXPOSED TO BLEOMYCIN
Autore:
PIPKIN JL; HINSON WG; FEUERS RJ; LYNCOOK LE; BURNS ER; DUFFY PH; HART R; CASCIANO DA;
Indirizzi:
US FDA,NATL CTR TOXICOL RES,DIV GENET TOXICOL,DGT,HFT 120 JEFFERSON AR 72079
Titolo Testata:
Aging
fascicolo: 2, volume: 6, anno: 1994,
pagine: 121 - 131
SICI:
0394-9532(1994)6:2<121:TTROSA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Keywords:
CALORIC RESTRICTION; RATS; STRESS PROTEINS 70 AND 90;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Pipkin et al., "THE TEMPORAL RELATIONSHIPS OF SYNTHESIS AND PHOSPHORYLATION IN STRESSPROTEINS 70 AND 90 IN AGED CALORIC RESTRICTED RATS EXPOSED TO BLEOMYCIN", Aging, 6(2), 1994, pp. 121-131

Abstract

A single intraperitoneal injection of the human therapeutic drug bleomycin (BL) was administered to three groups of male Fischer 344 rats at time 0, and the incorporation of [S-35]methionine (''synthesis'') and phosphorylation patterns of stress proteins (sps/hsps) from bone marrow cells were analyzed over time by two-dimensional electrophoresis and fluorography. Two groups of rats, young ad libitum (Y/AL - 3 months) and old ad libitum (O/AL - 28 months), had free access to rat chow, and a third group of old rats (O/CR - 28 months) were maintained on a caloric restricted intake (60% of the AL diet). The administration of BL in Y/AL, O/AL and O/CR animals activated the S-35-labeling of sp 90which reached a peak at 4 hours. Labeling of sp 90 was significantly greater in Y/AL compared to O/AL, and the incorporation pattern of O/CR was intermediate to Y/AL and O/AL animals. All labeling of sp 90 in each group had disappeared by 10 hours after BL administration. Stressprotein 70x (inducible form) in these three animal groups displayed asimilar pattern of S-35-incorporation, but the amount of labeling wasless than that of sp 90. No labeling of sp 70x remained by 13 hours after BL administration. Phosphorylation ([P-32] phosphate incorporation) of sp 90 reached a maximum level at 2 hours in all animals, and P-32-labeling in Y/AL was significantly increased over O/AL and O/CR withan intermediate level found in O/CR animals. The turnover rate (phosphorylation/dephosphorylation) of sp 90 induced by BL was significantlysuppressed and temporarily extended in O/AL as compared with O/CR, which implied that CR not only increased incorporation of sp 90, but also enhanced a utilization of the phosphate pool very similar to that seen in Y/AL animals.

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Documento generato il 01/04/20 alle ore 20:41:13