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Titolo:
IDENTIFICATION OF RESIDUES LINKED TO THE SLOW-]FAST TRANSITION OF THROMBIN
Autore:
GUINTO ER; VINDIGNI A; AYALA YM; DANG QD; DICERA E;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOPHYS,BOX 8231 ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOPHYS ST LOUIS MO 63110
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 24, volume: 92, anno: 1995,
pagine: 11185 - 11189
SICI:
0027-8424(1995)92:24<11185:IORLTT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECTED MUTAGENESIS; SPECIFICITY; SITE; PROTEINS; THROMBOMODULIN; FIBRINOGEN; HIRUDIN; DNA;
Keywords:
ALLOSTERY; FIBRINOGEN; HIRUDIN; MOLECULAR RECOGNITION; SITE-DIRECTED MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
E.R. Guinto et al., "IDENTIFICATION OF RESIDUES LINKED TO THE SLOW-]FAST TRANSITION OF THROMBIN", Proceedings of the National Academy of Sciences of the United Statesof America, 92(24), 1995, pp. 11185-11189

Abstract

Residues energetically linked to the allosteric transition of thrombin from its anticoagulant slow form to the procoagulant fast form have been identified by site-directed mutagenesis, The energetics of recognition by the two forms of the enzyme were probed by using a synthetic chromogenic substrate, fibrinogen, and hirudin, The thrombin residues E39, W60d, E192, D221, and D222 are linked to the slow --> fast transition and are part of an ''allosteric core'' through which events originating at the Na+ binding loop propagate to other regions of the enzyme, The thrombin residues Y76, W96, W148, and R173 lie at the peripheryof the allosteric core, affect recognition of fibrinogen and hirudin to the same extent in both forms, and are not linked to the slow. --> fast transition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:22:33