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Titolo:
RANDOM AMPLIFIED POLYMORPHIC DNA-FINGERPRINTING OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI ISOLATED FROM HUMAN FECES, SEAWATER AND POULTRY PRODUCTS
Autore:
HERNANDEZ J; FAYOS A; FERRUS MA; OWEN RJ;
Indirizzi:
UNIV POLITECN VALENCIA,DEPT BIOTECNOL,CAMINO DE VERA 14 E-46022 VALENCIA SPAIN CENT PUBL HLTH LAB,NATL COLLECT TYPE CULTURES LONDON NW9 5HT ENGLAND
Titolo Testata:
Research in microbiology
fascicolo: 8, volume: 146, anno: 1995,
pagine: 685 - 696
SICI:
0923-2508(1995)146:8<685:RAPDOC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA GENE PATTERNS; ARBITRARY PRIMERS; POLYMERASE; BIOTYPES; AMPLIFICATION; DIVERSITY; SEROTYPES; LARIDIS; STRAINS; SCHEME;
Keywords:
ENTERITIS, RAPD, CAMPYLOBACTER JEJUNI, CAMPYLOBACTER COLI, CAMPYLOBACTER LARI; GENETIC DIVERSITY, TYPING, EPIDEMIOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
J. Hernandez et al., "RANDOM AMPLIFIED POLYMORPHIC DNA-FINGERPRINTING OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI ISOLATED FROM HUMAN FECES, SEAWATER AND POULTRY PRODUCTS", Research in microbiology, 146(8), 1995, pp. 685-696

Abstract

The polymerase chain reaction (PCR) technique was used to obtain randomly amplified polymorphic DNA (RAPD) profiles from 64 type and serotype reference strains and 114 isolates of Campylobacter jejuni and C. coli from food, seawater and human faeces. Genetic diversity was detected among the strains asa total of 118 different RAPD profiles were obtained, each one containing from 4 to 11 bands between 0.30 and 1.50 kb. The discriminatory power of a random 10-mer primer (sequence 5'-CAATCGCCGT-3') was assessed. In general, no profiles were common to strains of the same Penner serogroup, but occasional strains from different Penner serotypes shared identical band profiles. RAPD analysis also differentiated between the species, and after numerical analysis, five main clusters were defined at the 40% similarity level, corresponding to C. jejuni, C. coil and C. lari with some exceptions. RAPD profiling of Campylobacter is highly discriminatory and is a valuable new alternative to traditional typing in epidemiological studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 23:06:09