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Titolo:
STIMULATION OF GLUT-1 GLUCOSE-TRANSPORTER EXPRESSION IN RESPONSE TO EXPOSURE TO CALCIUM IONOPHORE A-23187
Autore:
MITANI Y; BEHROOZ A; DUBYAK GR; ISMAILBEIGI F;
Indirizzi:
CASE WESTERN RESERVE UNIV,DEPT MED,DIV CLIN & MOLEC ENDOCRINOL,10900 EUCLID AVE CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DEPT MED,DIV CLIN & MOLEC ENDOCRINOL CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DEPT PHYSIOL & BIOPHYS,DIV CLIN & MOLEC ENDOCRINOL CLEVELAND OH 44106
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 5, volume: 38, anno: 1995,
pagine: 1228 - 1234
SICI:
0363-6143(1995)38:5<1228:SOGGEI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; CLONE-9 CELLS; MESSENGER-RNA; C-FOS; OXIDATIVE-PHOSPHORYLATION; SKELETAL-MUSCLE; TUMOR PROMOTER; INHIBITION; THAPSIGARGIN; TRANSCRIPTION;
Keywords:
GLUT-1 GENE TRANSCRIPTION; GLUCOSE TRANSPORT; ,2-BIS(2-AMINOPHENOXY)ETHANE-N,N,N',N'-TETRAACETIC ACID; CLONE 9 CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
Y. Mitani et al., "STIMULATION OF GLUT-1 GLUCOSE-TRANSPORTER EXPRESSION IN RESPONSE TO EXPOSURE TO CALCIUM IONOPHORE A-23187", American journal of physiology. Cell physiology, 38(5), 1995, pp. 1228-1234

Abstract

We tested the hypothesis that an increase in cytosolic calcium concentration stimulates glucose transporter isoform (GLUT-1) gene expression. Exposure of a rat liver cell line (Clone 9) to 3 mu M A-23187 for 12 h resulted in 3-, 5-, and 10-fold increases in cytochalasin B-inhibitable 3-O-methyl-D-glucose transport, GLUT-1 protein, and GLUT-1 mRNA content, respectively. The induction of GLUT-1 mRNA in response to A-23187 is not preceded by a significant decrease in cell ATP content. This induction is prevented by ,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid in conjunction with ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid. To investigate the mechanism of GLUT-1 mRNA induction, we found that exposure to A-23187 stabilized GLUT-1 mRNA: with the employment of actinomycin D, GLUT-1 mRNA had a half-life of 1.5 and 5.5 h in control and A-23187-treated cells, respectively. In nuclear run-on assays, the rate of GLUT-1 gene transcription was stimulated 1.5- to 1.7-fold in nuclei isolated from cells exposed toA-23187 for either 30 min or 2 h. These results demonstrate that exposure to A-23187 stimulates GLUT-1 gene expression and that the increase in GLUT-1 mRNA content is mediated in part by enhanced GLUT-1 gene transcription as well as decreased GLUT-1 mRNA degradation. The increase in GLUT-1 mRNA content, in turn, is associated with increased cell GLUT-1 content and enhanced glucose transport.

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Documento generato il 29/10/20 alle ore 12:49:36