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Titolo:
CLONING AND CHARACTERIZATION OF 2 RELATED SEROTONERGIC RECEPTORS FROMTHE BRAIN AND THE REPRODUCTIVE-SYSTEM OF APLYSIA THAT ACTIVATE PHOSPHOLIPASE-C
Autore:
LI XC; GIOT JF; KUHL D; HEN R; KANDEL ER;
Indirizzi:
COLUMBIA UNIV COLL PHYS & SURG,HOWARD HUGHES MED INST,722 W 168TH ST NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,HOWARD HUGHES MED INST NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,CTR NEUROBIOL & BEHAV NEW YORK NY 10032
Titolo Testata:
The Journal of neuroscience
fascicolo: 11, volume: 15, anno: 1995,
pagine: 7585 - 7591
SICI:
0270-6474(1995)15:11<7585:CACO2R>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; SPONTANEOUS TRANSMITTER RELEASE; TERM SYNAPTIC FACILITATION; PLEURAL SENSORY NEURONS; PRESYNAPTIC FACILITATION; GILL-WITHDRAWAL; CAMP; DISHABITUATION; POTENTIALS; MODULATION;
Keywords:
CLONING; APLYSIA; SEROTONIN; RECEPTOR; PHOSPHOLIPASE C; NERVOUS SYSTEM; REPRODUCTIVE SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
X.C. Li et al., "CLONING AND CHARACTERIZATION OF 2 RELATED SEROTONERGIC RECEPTORS FROMTHE BRAIN AND THE REPRODUCTIVE-SYSTEM OF APLYSIA THAT ACTIVATE PHOSPHOLIPASE-C", The Journal of neuroscience, 15(11), 1995, pp. 7585-7591

Abstract

Serotonin (5-HT) plays important roles in various behavioral and physiological processes in Aplysia californica. These include feeding, locomotion, circadian rhythm, learning and memory, synaptic plasticity, and synaptic growth. Serotonin modulates these various functions by interacting with different 5-HT receptor subtypes that are coupled to various second-messenger systems. We report here the isolation and characterization of the first two serotonergic receptors from Aplysia californica, Ap5-HTB1 and Ap5-HTB2, using a strategy based on the amino acidsequence homology among G-protein-coupled biogenic amine receptors. Ap5-HTB1 and Ap5-HTB2 are both intronless and highly homologous to eachother, sharing 79.5% sequence identity at the amino acid level. Sequence comparison reveals that these receptors are 33.1 to 23.3% identical to the following 5-HT receptors: 5-HTdro1, > 5-HT6 > 5-HTlym > mouse5-HT1B > 5-HTdro2A > mouse 5-HT7 > rat 5-HT2A. Both Ap5-HTB1 and Ap5-HTB2 encode functional 5-HT receptors. When expressed in cultured cells, these receptors stimulate phospholipase C in response to 5-HT in a dose-dependent manner. This stimulation can be blocked by specific 5-HT receptor antagonists. Using RT-PCR and Western blot analysis, we have detected these receptors in the CNS (Ap5-HTB2) and in the reproductive system (Ap5-HTB1). The nucleotide sequences of Ap5-HTB1 and Ap5-HTB2 were submitted to GenBank; the accession numbers are L43557 and L43558, respectively.

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Documento generato il 03/07/20 alle ore 16:33:24