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Titolo:
ISOLATION OF DAP3, A NOVEL MEDIATOR OF INTERFERON-GAMMA-INDUCED CELL-DEATH
Autore:
KISSIL JL; DEISS LP; BAYEWITCH M; RAVEH T; KHASPEKOV G; KIMCHI A;
Indirizzi:
WEIZMANN INST SCI,DEPT MOLEC GENET & VIROL IL-76100 REHOVOT ISRAEL WEIZMANN INST SCI,DEPT MOLEC GENET & VIROL IL-76100 REHOVOT ISRAEL
Titolo Testata:
The Journal of biological chemistry
fascicolo: 46, volume: 270, anno: 1995,
pagine: 27932 - 27936
SICI:
0021-9258(1995)270:46<27932:IODANM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOPTOSIS; PROTEIN; GENE; PATHWAYS; ENCODES; TOOL; P53;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Kissil et al., "ISOLATION OF DAP3, A NOVEL MEDIATOR OF INTERFERON-GAMMA-INDUCED CELL-DEATH", The Journal of biological chemistry, 270(46), 1995, pp. 27932-27936

Abstract

Interaction of certain cytokines with their corresponding cell-surface receptors induces programed cell death. Interferon-gamma induces in HeLa cells a type of cell death with features characteristic of programed cell death. Here, we report the isolation of a novel gene, DAP3 (death-associated protein-3), involved in mediating interferon-gamma-induced cell death. The rescue of this gene was performed by a functionalselection approach of gene cloning that is based on transfection withan antisense cDNA expression library, The antisense RNA-mediated inactivation of the DAPS gene protected the cells from interferon-gamma-induced cell death. This property endowed the cells expressing it with agrowth advantage in an environment restrictive due to the continuous presence of interferon-gamma and thus provided the basis of its selection, The gene is transcribed into a single 1.7-kilobase mRNA, which isubiquitously expressed in different tissues and codes for a 46-kDa protein carrying a potential P-loop motif. Ectopic expression of DAP3 inHeLa cells was not compatible with cell growth, resulting in a 16 fold reduction in the number of drug-resistant stable clones. The data presented suggest that DAP3 is a positive mediator of cell death inducedby interferon-gamma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:28:53