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Titolo:
IDENTIFICATION AND CHARACTERIZATION OF A NOVEL RELATED ADHESION FOCALTYROSINE KINASE (RAFTK) FROM MEGAKARYOCYTES AND BRAIN
Autore:
AVRAHAM S; LONDON R; FU YG; OTA S; HIREGOWDARA D; LI JZ; JIANG SX; PASZTOR LN; WHITE RA; GROOPMAN JE; AVRAHAM H;
Indirizzi:
HARVARD UNIV,DEACONESS HOSP,SCH MED,DEPT MED,DIV HEMATOL ONCOL,1 DEACONESS HOSP BOSTON MA 02215 UNIV MISSOURI,SCH MED,CHILDRENS MERCY HOSP,GENET SECT KANSAS CITY MO 64108
Titolo Testata:
The Journal of biological chemistry
fascicolo: 46, volume: 270, anno: 1995,
pagine: 27742 - 27751
SICI:
0021-9258(1995)270:46<27742:IACOAN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SWISS 3T3 CELLS; C-KIT LIGAND; SIGNAL TRANSDUCTION; SHORT ARM; PROTEIN; PHOSPHORYLATION; GENE; SRC; PP125(FAK); RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
S. Avraham et al., "IDENTIFICATION AND CHARACTERIZATION OF A NOVEL RELATED ADHESION FOCALTYROSINE KINASE (RAFTK) FROM MEGAKARYOCYTES AND BRAIN", The Journal of biological chemistry, 270(46), 1995, pp. 27742-27751

Abstract

We have isolated a cDNA encoding a novel human intracytoplasmic tyrosine kinase, termed RAFTK (for a related adhesion focal tyrosine kinase), In addition, we have cloned and characterized the murine homolog ofthe human RAFTK cDNA, Comparison of the deduced amino acid sequences of human RAFTK and murine Raftk cDNAs revealed 95% homology, indicating that RAFTK is highly conserved between these species, The RAFTK cDNAclone, encoding a polypeptide of 1009 amino acids, has closest homology (48% identity, 65% similarity) to the focal adhesion kinase (pp125(FAK)). Comparison of the deduced amino acid sequences also indicates that RAFTK, like pp125(FAK), lacks a transmembrane region, myristylation sites, and SH2 and SH3 domains, In addition, like pp125(FAK), RAFTK contains a kinase domain flanked by large N-terminal (426 residues) and C-terminal (331 residues) domains, and the C-terminal region contains a predicted proline-rich stretch of residues, In fetal tissues, RAFTK expression was abundant in brain, and low levels were observed in lung and liver. In adult tissues, it was less restricted, indicating that RAFTK expression is developmentally up-regulated, Expression of RAFTK was also observed in human CD34(+) marrow cells, primary bone marrowmegakaryocytes, platelets, and various areas of brain, The human RAFTK gene was assigned to human chromosome 8 using genomic DNAs from human/rodent somatic cell hybrid lines, The mouse Raftk gene was mapped tochromosome 14, closely linked to gonadotropin-releasing hormone, Using specific antibodies for RAFTK, a similar to 123-kDa protein from thehuman megaIzaryocytic CMK cell line was immunoprecipitated. Treatmentof the megakaryocytic CMK cells with thrombin caused a rapid induction of tyrosine phosphorylation of RAFTK protein, The structural features of RAFTK suggest that it is a member of the focal adhesion kinase gene family and may participate in signal transduction in human megakaryocytes and brain as well as in other cell types.

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Documento generato il 15/07/20 alle ore 08:42:25