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Titolo:
THE ADENOVIRUS PROTEASE IS REQUIRED FOR VIRUS ENTRY INTO HOST-CELLS
Autore:
COTTEN M; WEBER JM;
Indirizzi:
RES INST MOLEC PATHOL,DR BOHR GASSE 7 A-1030 VIENNA AUSTRIA UNIV SHERBROOKE,FAC MED,DEPT MICROBIOL SHERBROOKE PQ J1H 5N4 CANADA
Titolo Testata:
Virology
fascicolo: 2, volume: 213, anno: 1995,
pagine: 494 - 502
SICI:
0042-6822(1995)213:2<494:TAPIRF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYLYSINE DNA COMPLEXES; MEDIATED GENE DELIVERY; EARLY REGION-4; TYPE-2; PROTEINASE; PARTICLES; VIRION; EXPRESSION; PEPTIDE; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M. Cotten e J.M. Weber, "THE ADENOVIRUS PROTEASE IS REQUIRED FOR VIRUS ENTRY INTO HOST-CELLS", Virology, 213(2), 1995, pp. 494-502

Abstract

We have analyzed the mechanisms used by adenovirus to gain entry intothe host cell. Using both virus infection and the ability of adenovirus particles to enhance polylysine/DNA uptake as a measure of virus entry, we have demonstrated that the adenovirus-encoded 23K protease is required for two functions in the infection process. A proteolytic processing of the capsid is required to generate a Virus capsid that can increase membrane interactions at pH 5. We have found that forms of adenovirus capsid that have not undergone the processing reactions (immature capsids) are deficient in their ability to disrupt membranes at pH 5 and are unable to enhance the entry of polylysine/DNA complexes. Asecond role of the protease was revealed by experiments using inhibitors of the protease, Mature virus capsids lose their ability to enhance gene delivery and become noninfectious after exposure to inhibitors of the protease (1 mu M N-ethylmaleimide, 100-300 PM copper chloride, 1 mu M MDL28170, or anti-protease antiserum), suggesting that the viral protease activity is required during the cellular entry process. (C)1995 Academic Press. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:59:44