Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
2 STRONG 5'-SPLICE SITES AND COMPETING, SUBOPTIMAL 3'-SPLICE SITES INVOLVED IN ALTERNATIVE SPLICING OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA
Autore:
OREILLY MM; MCNALLY MT; BEEMON KL;
Indirizzi:
JOHNS HOPKINS UNIV,DEPT BIOL,3400 N CHARLES ST BALTIMORE MD 21218 JOHNS HOPKINS UNIV,DEPT BIOL BALTIMORE MD 21218 MED COLL WISCONSIN,DEPT MICROBIOL MILWAUKEE WI 53226
Titolo Testata:
Virology
fascicolo: 2, volume: 213, anno: 1995,
pagine: 373 - 385
SICI:
0042-6822(1995)213:2<373:2S5SAC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
REV GENE-PRODUCT; LATE REPLACEMENT VECTOR; VIRAL MESSENGER-RNA; PROTEIN EXPRESSION; INTRONIC SEQUENCES; ENV; CIS; RETROVIRUS; INFECTION; TAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Oreilly et al., "2 STRONG 5'-SPLICE SITES AND COMPETING, SUBOPTIMAL 3'-SPLICE SITES INVOLVED IN ALTERNATIVE SPLICING OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA", Virology, 213(2), 1995, pp. 373-385

Abstract

The human immunodeficiency virus type 1 (HIV-I) genome contains 20 exons that are alternatively spliced from 16 splice sites to generate more than 40 different mRNAs, including incompletely spliced and unspliced mRNAs. In contrast to avian retroviral RNA, which has a cis-acting element in gag that negatively regulates splicing (NRS), HIV-I RNA didnot have any NRS sequences in the gag or pol genes detectable by a splicing inhibition assay. However, this assay demonstrated that the HIV-1 first 5' splice site competed with a cellular 5' splice site, suggesting that HIV-1 may have some strong splice sites. To extend this observation, we used a splice site swapping strategy to determine the efficiency of 14 HIV-I splice sites in human beta globin chimeras tested in transient transfection experiments. While the Ist HIV-1 5' splice site used in all spliced transcripts and the 4th 5' splice site used inmost of the 2-kb transcripts were efficient, the other splice sites, including all the 3' splice sites; were less efficient, ranging in usefrom 25 to 60%. We propose that this range of splice site efficiencies contributes to the regulation of alternative splicing of HIV-1 mRNAs. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 06:35:20