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Titolo:
SIMULTANEOUS DETERMINATION OF PLASMA PHENYTOIN AND ITS PRIMARY HYDROXYLATED METABOLITES IN CARBON TETRACHLORIDE-INTOXICATED RATS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
TANAKA E; SAKAMOTO N; INUBUSHI M; MISAWA S;
Indirizzi:
UNIV TSUKUBA,INST COMMUNITY MED TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 1, volume: 673, anno: 1995,
pagine: 147 - 151
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROXYPHENYTOIN; URINE;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
7
Recensione:
Indirizzi per estratti:
Citazione:
E. Tanaka et al., "SIMULTANEOUS DETERMINATION OF PLASMA PHENYTOIN AND ITS PRIMARY HYDROXYLATED METABOLITES IN CARBON TETRACHLORIDE-INTOXICATED RATS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Journal of chromatography B. Biomedical applications, 673(1), 1995, pp. 147-151

Abstract

We have developed a simple and sensitive method for the simultaneous determination of phenytoin (PHT), 5-(p-hydroxyphenyl)-5-phenylhydantoin (P-HPPH) and 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) in rat plasma by high-performance liquid chromatography. The three substances were separated on a reversed-phase column (5 mu m TSK gel ODS-80TM, 250 mm x 4.6 mm I.D.) using acetonitrile-0.008 M NaH2PO4 (pH 6) (35:65, v/v) as a mobile phase at a flow-rate of 0.8 ml/min. Absorbance was monitored at 215 nm. The quantification limit was 50 ng/ml for each of PHT, m-HPPH and p-HPPH. The mean recoveries for DPH, m-HPPH and p-HPPH from plasma were 95.6 +/- 3.6, 94.5 +/- 4.2 and 98.6 +/- 2.9%, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 09:56:07