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Titolo:
EXPRESSION OF THE MACROPHAGE SCAVENGER RECEPTOR IN ATHEROMA - RELATIONSHIP TO IMMUNE ACTIVATION AND THE T-CELL CYTOKINE INTERFERON-GAMMA
Autore:
GENG YJ; HOLM J; NYGREN S; BRUZELIUS M; STEMME S; HANSSON GK;
Indirizzi:
KAROLINSKA INST,KING GUSTAF V RES INST S-17176 STOCKHOLM SWEDEN KAROLINSKA INST,KING GUSTAF V RES INST S-17176 STOCKHOLM SWEDEN GOTHENBURG UNIV,DEPT SURG S-41124 GOTHENBURG SWEDEN GOTHENBURG UNIV,DEPT LAB MED S-41124 GOTHENBURG SWEDEN
Titolo Testata:
Arteriosclerosis, thrombosis, and vascular biology
fascicolo: 11, volume: 15, anno: 1995,
pagine: 1995 - 2002
SICI:
1079-5642(1995)15:11<1995:EOTMSR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; LOW-DENSITY-LIPOPROTEIN; HUMAN ATHEROSCLEROTIC PLAQUES; HUMAN MONOCYTE-MACROPHAGES; LYMPHOCYTES-T; ENDOTHELIAL-CELLS; GENE-EXPRESSION; MESSENGER-RNA; LESIONS; ALPHA;
Keywords:
ATHEROSCLEROSIS; SCAVENGER RECEPTOR; LDL; LDL OXIDATION; MACROPHAGES; T LYMPHOCYTES; IMMUNOHISTOCHEMISTRY; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
Y.J. Geng et al., "EXPRESSION OF THE MACROPHAGE SCAVENGER RECEPTOR IN ATHEROMA - RELATIONSHIP TO IMMUNE ACTIVATION AND THE T-CELL CYTOKINE INTERFERON-GAMMA", Arteriosclerosis, thrombosis, and vascular biology, 15(11), 1995, pp. 1995-2002

Abstract

Scavenger receptors mediate internalization of modified lipoproteins and foam cell transformation of monocyte-derived macrophages. Their expression is independent of the intracellular cholesterol content but is modulated by immune-derived cytokines. We investigated macrophage scavenger receptor (MSR) expression in monocyte-macrophages from human peripheral blood and in atherosclerotic lesions and analyzed its relationship to T lymphocytes and immunoregulatory cytokines by immunohistochemistry and polymerase chain reaction (PCR). Antibodies specific for the two MSR. isoforms were generated by immunizing rabbits with isoform-specific synthetic peptides conjugated to keyhole limpet hemocyanin. In human atherosclerotic plaques, these antibodies stained macrophages and foam cells in a pattern that corresponded to the distribution ofthe macrophage marker CD68. CDS-positive T cells and alpha-actin-positive smooth muscle cells exhibited no reactivity to the anti-MSR antibodies. The frequency of cells stained with antibodies to MSR type I was equal to that of cells stained for type II, suggesting that most macrophages coexpress both isoforms. Reverse transcription (RT)-PCR analysis confirmed that both MSR isoforms were expressed in all plaques examined. There was, however, a tendency toward a lower immunohistochemical staining intensity for MSR type I and a decreased number of lipid-rich foam cells in T cell-rich areas. The mRNAs for interleukin-2 and interferon-gamma, two major products of activated T cells, were detected by RT-PCR in all plaques tested. This indicates that activation of Tlymphocytes occurs in atherosclerotic plaques. Since interferon-gammadownregulates MSR expression, these observations suggest a potential mechanism for local regulation of MSR expression in the atherosclerotic plaque.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/02/20 alle ore 12:53:42