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Titolo:
BIOSYNTHESIS OF PROSTAGLANDIN D-2 BY MOTONEURONS AND DORSAL HORN MICRONEURONS - A BIOCHEMICAL AND HIGH-RESOLUTION IMMUNOCYTOCHEMICAL STUDY IN CHICK SPINAL-CORD
Autore:
VESIN MF; BILLOTTE C; DROZ B;
Indirizzi:
FAC MED LAUSANNE,INST HISTOL & EMBRYOL,RUE BUGNON 9 CH-1005 LAUSANNE SWITZERLAND
Titolo Testata:
Neuroscience
fascicolo: 3, volume: 69, anno: 1995,
pagine: 967 - 975
SICI:
0306-4522(1995)69:3<967:BOPDBM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID METABOLITES; RAT-BRAIN; D SYNTHETASE; SUBARACHNOID HEMORRHAGE; CEREBRAL-ISCHEMIA; FATTY-ACIDS; D SYNTHASE; REPERFUSION; INJURY; CYCLOOXYGENASE;
Keywords:
CENTRAL NERVOUS SYSTEM; PROSTAGLANDIN D SYNTHASE; ELECTRON MICROSCOPY; ROUGH ENDOPLASMIC RETICULUM; RIBOSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
M.F. Vesin et al., "BIOSYNTHESIS OF PROSTAGLANDIN D-2 BY MOTONEURONS AND DORSAL HORN MICRONEURONS - A BIOCHEMICAL AND HIGH-RESOLUTION IMMUNOCYTOCHEMICAL STUDY IN CHICK SPINAL-CORD", Neuroscience, 69(3), 1995, pp. 967-975

Abstract

Prostaglandin D-2 is one of the major prostanoids formed from [C-14]arachidonic acid by the central nervous system. The aim of the present study is to specify the prostaglandin D-2 biosynthetic capacity in thechick spinal cord and to identify the ceil type involved in this synthesis. A highly specific and sensitive enzyme immunoassay allowed us to demonstrate that the amount of newly formed prostaglandin D-2 increases proportionally with the concentration of free arachidonic acid of either exogenous or endogenous origin and reaches concentration valuesranging from 10(-9) to 10(-6) M. The sites of prostaglandin D-2 synthesis were localized in Vibratome sections of spinal cord after incubation with antibodies raised against glutathione-independent prostaglandin D synthase; controls were performed with anti-glutathione-dependentprostaglandin D synthase antibodies and non-immune rabbit or goat serum. After immunoprocessing, electron microscope examination revealed that the specific immunoreactivity was confined to small neurons of laminae II and III in the dorsal horn and to motoneurons in the ventral horn of the spinal cord. The immunodeposits were associated with rough endoplasmic reticulum profiles distributed throughout the dorsal horn neurons or restricted to limited subsurface areas of perikarya and dendrites in motoneurons. Since the immunoreactive neurons in the dorsal horn were closely related to blood capillaries, prostaglandin D-2 may be suspected to play a role in the regulation of the microcirculation. The accumulation of prostaglandin D synthase in motoneuron areas facing astrocytic membrane stacks suggests that prostaglandin D-2 could interact with astrocytic functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 07:51:06