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Titolo:
VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR DIRECTLY QUANTIFYING THE ENANTIOMERS OF MDL-74156, THE PRIMARY METABOLITE OF DOLASETRON MESYLATE, IN HUMAN PLASMA
Autore:
MCELVAIN JS; VANDIVER VJ; EICHMEIER LS;
Indirizzi:
HOECHST MARION ROUSSEL INC,DEPT BIOANALYT,POB 9627 KANSAS CITY MO 64131
Titolo Testata:
Journal of pharmaceutical and biomedical analysis
fascicolo: 4, volume: 15, anno: 1997,
pagine: 513 - 521
SICI:
0731-7085(1997)15:4<513:VOARHM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; 5-HT3; PHARMACOKINETICS; ANTAGONIST; MESILATE;
Keywords:
DIRECT CHIRAL REVERSED-PHASE HPLC; DOLASETRON MESYLATE; MDL 74 156; PLASMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
J.S. Mcelvain et al., "VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR DIRECTLY QUANTIFYING THE ENANTIOMERS OF MDL-74156, THE PRIMARY METABOLITE OF DOLASETRON MESYLATE, IN HUMAN PLASMA", Journal of pharmaceutical and biomedical analysis, 15(4), 1997, pp. 513-521

Abstract

A direct chiral HPLC method has been developed and validated to quantitate the enantiomers of MDL 74 156, the primary metabolite of dolasetron mesylate, in human plasma over the concentration range 1.70-340 ngml(-1) Dolasetron mesylate is a 5-HT3 receptor antagonist that is currently being developed as an antiemetic. Both enantiomers of MDL 74156and the internal standard (granisetron) were first extracted from alkanized plasma using methyl t-butyl ether. The analytes were then back-extracted into formic acid, separated on a ovomucoid-bonded HPLC column, and detected by native fluorescence (excitation wavelength of 274 nm and emission wavelength of 345 nm). The completed validation demonstrated the method to be accurate, precise, and specific for the direct quantitation of MDL 74156 enantiomers in human plasma. This procedure has been used on a routine basis to quantify the relative concentrations of each enantiomer of MDL 74 156 in both oral and intravenous pharmacokinetic studies of dolasetron mesylate in normal human volunteers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:55:38