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Titolo:
FOLLOWING CELL FATE IN THE LIVING MOUSE EMBRYO
Autore:
ZERNICKAGOETZ M; PINES J; HUNTER SM; DIXON JPC; SIEMERING KR; HASELOFF J; EVANS MJ;
Indirizzi:
WELLCOME CRC INST,TENNIS COURT RD CAMBRIDGE CB2 1QR ENGLAND UNIV CAMBRIDGE,DEPT GENET CAMBRIDGE CB2 1TN ENGLAND UNIV CAMBRIDGE,DEPT ZOOL CAMBRIDGE CB2 1TN ENGLAND MRC,MOL BIOL LAB CAMBRIDGE ENGLAND
Titolo Testata:
Development
fascicolo: 6, volume: 124, anno: 1997,
pagine: 1133 - 1137
SICI:
0950-1991(1997)124:6<1133:FCFITL>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN-FLUORESCENT PROTEIN; LIVE ZEBRAFISH EMBRYOS; GFP; EXPRESSION; GENE; TOOL;
Keywords:
GREEN FLUORESCENT PROTEIN; GFP; CELL FATE; MOUSE DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
M. Zernickagoetz et al., "FOLLOWING CELL FATE IN THE LIVING MOUSE EMBRYO", Development, 124(6), 1997, pp. 1133-1137

Abstract

It has been difficult to follow many of the dramatic changes in cell fate and cell migration during mouse development. This is because there has been no enduring marker that would allow cells to be recognised in the living embryo. We believe that we have overcome this problem bydeveloping a novel form of green fluorescent protein, named MmGFP, that proves to be easily visible and non toxic to mouse cells and does not perturb embryogenesis. We show that synthetic mRNA encoding MmGFP can be injected into blastomeres to follow the fate of their progeny during preimplantation development, We have made a stable embryonic stemcell line that expresses MmGFP and introduced these fluorescent cellsinto mouse embryos. For the first time, we have been able to follow the fate of embryonic stem cells in living embryos and to observe directly the contribution of these cells to distinct lineages of the postimplantation embryo. This approach should lead to a more complete description of the dynamics of cell fate in the mouse.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:30:33