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Titolo:
IDENTIFICATION OF PRODUCTS FORMED BY A FRUCTAN-FRUCTAN FRUCTOSYLTRANSFERASE ACTIVITY FROM LOLIUM-RIGIDUM
Autore:
STJOHN JA; SIMS IM; BONNETT GD; SIMPSON RJ;
Indirizzi:
CSIRO,DIV PLANT IND,GPO BOX 1600 CANBERRA ACT 2601 AUSTRALIA CSIRO,DIV PLANT IND CANBERRA ACT 2601 AUSTRALIA UNIV MELBOURNE,DEPT AGR & RESOURCE MANAGEMENT PARKVILLE VIC 3052 AUSTRALIA UNIV MELBOURNE,PLANT CELL BIOL RES CTR,DEPT BOT PARKVILLE VIC 3052 AUSTRALIA
Titolo Testata:
New phytologist
fascicolo: 2, volume: 135, anno: 1997,
pagine: 249 - 257
SICI:
0028-646X(1997)135:2<249:IOPFBA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASPARAGUS-OFFICINALIS L; FRUCTOSYL TRANSFERASE; EXCISED LEAVES; PURIFICATION; ROOTS; 6G-FRUCTOSYLTRANSFERASE; BIOSYNTHESIS; TEMULENTUM;
Keywords:
LOLIUM RIGIDUM; FRUCTAN-FRUCTAN FRUCTOSYLTRANSFERASE; OLIGOFRUCTAN; FRUCTAN SYNTHESIS; FRUCTAN STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Stjohn et al., "IDENTIFICATION OF PRODUCTS FORMED BY A FRUCTAN-FRUCTAN FRUCTOSYLTRANSFERASE ACTIVITY FROM LOLIUM-RIGIDUM", New phytologist, 135(2), 1997, pp. 249-257

Abstract

The products formed by a fructan:fructan fructosyltransferase (FFT) activity purified from Lolium rigidum Gaudin were identified after gas chromatography-mass spectrometry of partially methylated alditol acetates, electrospray ionization-mass spectrometry and reversed-phase high-performance liquid chromatography. The FFT activity synthesized oligofructans up to degree of polymerization (DP) 6, but did not synthesizefructans of DP > 6 even when assayed with (1,1,1)-kestopentaose for up to 10 h. The FFT activity when assayed with 1-kestose or 6(G)-kestose synthesized fructan with fructosyl residues almost exclusively linked by beta-2,1-glycosidic linkages. When assayed with 1-kestose, the FFT activity synthesized tetrasaccharides and pentasaccharides with an internal glucosyl residue. The predominant tetrasaccharide was (1&6(G))-kestotetraose and the predominant pentasaccharide was (1&6(G),1)-kestopentaose. By comparison, tetrasaccharides and pentasaccharides extracted from L. rigidum also contained predominantly beta-2,1-glycosidic linked fructans with an internal glucosyl residue. The only exception was that one of the pentasaccharides contained beta-2,1- and beta-2,6-glycosidic linked fructosyl residues. This pentasaccharide was not synthesized by the FFT activity. The role of this FFT activity in formation of oligofructans in L. rigidum is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:40:28