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Titolo:
DIFFERENCES IN THE LOCALIZATION OF THE POSTSYNAPTIC NITRIC-OXIDE SYNTHASE-I AND ACETYLCHOLINESTERASE SUGGEST A HETEROGENEITY OF NEUROMUSCULAR-JUNCTIONS IN RAT AND MOUSE SKELETAL-MUSCLES
Autore:
GROZDANOVIC Z; CHRISTOVA T; GOSSRAU R;
Indirizzi:
FREE UNIV BERLIN,CLIN BENJAMIN FRANKLIN,DEPT ANAT,KONIGIN LUISE STR 15 D-14195 BERLIN GERMANY FREE UNIV BERLIN,CLIN BENJAMIN FRANKLIN,DEPT ANAT D-14195 BERLIN GERMANY MED UNIV,DEPT ANAT & HISTOL BG-1431 SOFIA BULGARIA
Titolo Testata:
Acta histochemica
fascicolo: 1, volume: 99, anno: 1997,
pagine: 47 - 53
SICI:
0065-1281(1997)99:1<47:DITLOT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMATE-LIKE IMMUNOREACTIVITY; NADPH-DIAPHORASE; SPINAL-CORD; HISTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; NEURONS; SARCOLEMMA; EXPRESSION; NOS;
Keywords:
NITRIC OXIDE SYNTHASE I; NEUROMUSCULAR JUNCTION; SKELETAL MUSCLE; MDX MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
Z. Grozdanovic et al., "DIFFERENCES IN THE LOCALIZATION OF THE POSTSYNAPTIC NITRIC-OXIDE SYNTHASE-I AND ACETYLCHOLINESTERASE SUGGEST A HETEROGENEITY OF NEUROMUSCULAR-JUNCTIONS IN RAT AND MOUSE SKELETAL-MUSCLES", Acta histochemica, 99(1), 1997, pp. 47-53

Abstract

Recently, nitric oxide synthase (NOS)I has been identified in skeletal muscle fibers, where the enzyme is found to be associated to the sarcolemma by the alpha 1-syntrophin-dystrophin complex. It has, however,been proposed that a substantial proportion of NOS I at the neuromuscular junction (NMJ) is of neuronal origin. We have, therefore, investigated the distribution of NOS I in NMJ of normal rats and mice as wellas mdx mice which lack dystrophin and, consequently, NOS I in the sarcolemma region by enzyme histochemical and immunohistochemical techniques. Sites of NOS I accumulation, evident at NMJ of healthy animals, were absent in mdx mice, indicating a predominently, if not exclusively, postsynaptic localization of NOSI at NMJ. Moreover, simultaneous demonstration of acetylcholinesterase (AChE) activity revealed a heterogeneity of NMJ in rat and mouse skeletal muscles: type I showed only AChE activity and was found to predominate; type II was spatially separated from the AChE-positive NMJ, occurred less frequently and contained both AChE activity and NOS I. These data suggest that type II NMJ are provided with additional regulatory mechanisms, such as free radical signaling by the NOS I-derived NO which may exert modulatory effects onthe choline acetyltransferase/ACh/AChE pathway. Furthermore, type II may represent those NMJ where recently glutamate-gated NMDA-type Ca2+ channels have been described, which in analogy to those in the nervoussystem may serve also in skeletal muscle fibers as NOS I activators.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:24:08