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Titolo:
MOLECULAR-DETECTION OF STREPTOMYCIN-PRODUCING STREPTOMYCETES IN BRAZILIAN SOILS
Autore:
HUDDLESTON AS; CRESSWELL N; NEVES MCP; BERINGER JE; BAUMBERG S; THOMAS DI; WELLINGTON EMH;
Indirizzi:
UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND UNIV WARWICK,DEPT BIOL SCI COVENTRY CV4 7AL W MIDLANDS ENGLAND UNIV BRISTOL,DEPT BIOL SCI BRISTOL BS8 1UG AVON ENGLAND UNIV LEEDS,DEPT GENET LEEDS LS2 9JT W YORKSHIRE ENGLAND CNPBS,CTR NACL PESQUISA BIOL SOLO,EMBRAPA BR-23851970 RIO JANEIRO BRAZIL
Titolo Testata:
Applied and environmental microbiology
fascicolo: 4, volume: 63, anno: 1997,
pagine: 1288 - 1297
SICI:
0099-2240(1997)63:4<1288:MOSSIB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBIOTIC-RESISTANCE; IDENTIFICATION; BACTERIA; PLASMID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Huddleston et al., "MOLECULAR-DETECTION OF STREPTOMYCIN-PRODUCING STREPTOMYCETES IN BRAZILIAN SOILS", Applied and environmental microbiology, 63(4), 1997, pp. 1288-1297

Abstract

Actinomycetes were isolated from soybean rhizosphere soil collected at two field sites in Brazil, All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains were streptomycin resistant. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains fi om cultivated sites being placed in cluster 1, Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin-producing streptomycetes were phylogenetically diverse, It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:16:42