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Titolo:
MAC-1 (CDLLB CD18) IS AN OLIGODEOXYNUCLEOTIDE-BINDING PROTEIN/
Autore:
BENIMETSKAYA L; LOIKE JD; KHALED Z; LOIKE G; SILVERSTEIN SC; CAO L; KHOURY JE; CAI TQ; STEIN CA;
Indirizzi:
COLUMBIA UNIV COLL PHYS & SURG,630 W 168TH ST NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG NEW YORK NY 10032 ROCKEFELLER UNIV,CELLULAR PHYSIOL & IMMUNOL LAB NEW YORK NY 10021
Titolo Testata:
Nature medicine
fascicolo: 4, volume: 3, anno: 1997,
pagine: 414 - 420
SICI:
1078-8956(1997)3:4<414:M(CIAO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES; ANTISENSE OLIGODEOXYNUCLEOTIDES; TYROSINE PHOSPHORYLATION; OLIGONUCLEOTIDE UPTAKE; SCAVENGER RECEPTORS; MOUSE MODEL; HL-60 CELLS; I-DOMAIN; CD11B/CD18;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
L. Benimetskaya et al., "MAC-1 (CDLLB CD18) IS AN OLIGODEOXYNUCLEOTIDE-BINDING PROTEIN/", Nature medicine, 3(4), 1997, pp. 414-420

Abstract

We have studied the interactions of phosphodiester and phosphorothioate oligodeoxynucleotides with Mac-1 (CD11b/CD18; alpha M beta 2), a heparin-binding integrin found predominately on the surface of polymorphonuclear leukocytes (PMNs), macrophages and natural killer cells. Binding of a homopolymer of thymidine occurred on both the alpha M and beta 2 subunits. Soluble fibrinogen, a natural ligand for Mac-1, was an excellent competitor of the binding of a phosphorothioate oligodeoxynucleotide to both TNF-alpha-activated and nonactivated PMNs. Upregulation of cell-surface Mac-1 expression increased cell-surface binding of oligodeoxynucleotides. Binding was inhibited by anti-Mac-1 monoclonal antibodies, and the increase in cell-surface binding was correlated with a three- to fourfold increase in internalization by PMNs. An oligodeoxynucleotide inhibited beta 2-dependent migration through Matrigel, but the production of reactive oxygen species in PMNs adherent to fibrinogen dramatically increased. Thus, our data demonstrate that Mac-1 isa cell-surface receptor for oligodeoxynucleotides that can mediate their internalization and that this binding may have important functional consequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 12:51:24