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Titolo:
CRYOPRESERVATION OF SPERMATOZOA OF THE EDIBLE MUD CRAB SCYLLA-SERRATA(FORSKAL)
Autore:
BHAVANISHANKAR S; SUBRAMONIAM T;
Indirizzi:
UNIV MADRAS,DEPT ZOOL,UNIT INVERTEBRATE REPROD,GUINDY CAMPUS MADRAS 600025 TAMIL NADU INDIA
Titolo Testata:
The Journal of experimental zoology
fascicolo: 4, volume: 277, anno: 1997,
pagine: 326 - 336
SICI:
0022-104X(1997)277:4<326:COSOTE>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; SICYONIA-INGENTIS; LIVING CELLS; SPERM; CRYOPROTECTANTS; A23187; VIABILITY; INTEGRITY; INDUCTION; BRACHYURA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
S. Bhavanishankar e T. Subramoniam, "CRYOPRESERVATION OF SPERMATOZOA OF THE EDIBLE MUD CRAB SCYLLA-SERRATA(FORSKAL)", The Journal of experimental zoology, 277(4), 1997, pp. 326-336

Abstract

Biotechnique for programmed freezing of the spermatozoa of the ediblemud crab Scylla serrata and the procedures for assessing viability ofthe frozen-thawed spermatozoa are described. Free spermatozoa recovered from spermathecae and those released from the spermatophores were subjected to cryoprotectant treatment at different physiological temperatures (30 degrees C, 23 degrees C, 15 degrees C), frozen to subzero temperatures in a rate-controlled programmable freezer Kryo 10/1.7 and subsequently plunged into liquid nitrogen. Post-thaw viability, after 8 hr of storage at -196 degrees C, was assessed by artificial induction of acrosome reaction using divalent cation ionophore A23187. Hypo/hyperosmotic sensitivity tests were used to assess the membrane integrity of the spermatozoa before and after cryopreservation experiments. Viability tests using dye exclusion methods were discontinued owing to variations in incorporation of different stains into the spermatozoa. The limitations of the dye exclusion method as an indicator of sperm viability are discussed. Dimethyl sulfoxide, ethylene glycol and glycerol offered cryoprotection at concentrations ranging from 5% to 15% v/v. Post-thaw survival was highest (52%) with 12.5% glycerol at a coolingrate of -5 degrees C/min. (C) l997 Wiley-Liss,Inc.

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Documento generato il 02/07/20 alle ore 22:10:16