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Titolo:
CRYO-EM VISUALIZATION OF AN EXPOSED RGD EPITOPE ON ADENOVIRUS THAT ESCAPES ANTIBODY NEUTRALIZATION
Autore:
STEWART PL; CHIU CY; HUANG S; MUIR T; ZHAO YM; CHAIT B; MATHIAS P; NEMEROW GR;
Indirizzi:
SCRIPPS CLIN & RES INST,DEPT IMMUNOL LA JOLLA CA 92037 SCRIPPS CLIN & RES INST,DEPT IMMUNOL LA JOLLA CA 92037 UNIV CALIF LOS ANGELES,SCH MED,DEPT MOL & MED PHARMACOL,CRUMP INST BIOL IMAGING LOS ANGELES CA 00000 ROCKEFELLER UNIV,MASS SPECTROMETRY LAB NEW YORK NY 10021
Titolo Testata:
EMBO journal
fascicolo: 6, volume: 16, anno: 1997,
pagine: 1189 - 1198
SICI:
0261-4189(1997)16:6<1189:CVOAER>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUTH-DISEASE VIRUS; EPIDERMAL GROWTH-FACTOR; X-RAY CRYSTALLOGRAPHY; PENTON-BASE; CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL STRUCTURE; ELECTRON-MICROSCOPY; GENE-TRANSFER; HELA-CELLS; PROTEIN;
Keywords:
ADENOVIRUS; CRYOELECTRON MICROSCOPY; IMAGE RECONSTRUCTION; INTEGRINS; NEUTRALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
P.L. Stewart et al., "CRYO-EM VISUALIZATION OF AN EXPOSED RGD EPITOPE ON ADENOVIRUS THAT ESCAPES ANTIBODY NEUTRALIZATION", EMBO journal, 16(6), 1997, pp. 1189-1198

Abstract

Interaction of the adenovirus penton base protein with av integrins promotes virus entry into host cells, The location of the integrin binding sequence Arg-Gly-Asp (RGD) on human type 2 adenovirus (Ad2) was visualized by cryo-electron microscopy (cryo-EM) and image reconstruction using a mAb (DAV-1) which recognizes a linear epitope, IRGDTFATR, The sites for DAV-1 binding corresponded to the weak density above each of the five 22 Angstrom protrusions on the adenovirus penton base protein. Modeling of a Fab fragment crystal structure into the adenovirus-Fab cryo-EM density indicated a large amplitude of motion for the Fab and the RGD epitope. An unexpected finding was that Fab fragments, butnot IgG antibody molecules, inhibited adenovirus infection, Steric hindrance from the adenovirus fiber and a few bound IgG molecules, as well as epitope mobility, most likely prevent binding of IgG antibodies to all five RGD sites on the penton base protein within the intact virus, These studies indicate that the structure of the adenovirus particle facilitates interaction with cell integrins, whilst restricting binding of potentially neutralizing antibodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 12:51:45