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Titolo:
ISOLATION AND CHARACTERIZATION OF LIVER EPITHELIAL-CELL LINES FROM WILD-TYPE AND MUTANT TGN737RPW MICE
Autore:
RICHARDS WG; YODER BK; ISFORT RJ; DETILLEUX PG; FOSTER C; NEILSEN N; WOYCHIK RP; WILKINSON JE;
Indirizzi:
UNIV TENNESSEE,COLL VET MED,DEPT PATHOBIOL,POB 1071 KNOXVILLE TN 37901 UNIV TENNESSEE,SCH VET MED,DEPT PATHOBIOL KNOXVILLE TN 00000 UCB PHARMA CHEMIN DU FORRIEST BRAIN BELGIUM PROCTER & GAMBLE CO,MIAMI VALLEY LAB,HSD,CP&RSD CINCINNATI OH 00000 OAK RIDGE NATL LAB,DIV BIOL OAK RIDGE TN 37831
Titolo Testata:
The American journal of pathology
fascicolo: 4, volume: 150, anno: 1997,
pagine: 1189 - 1197
SICI:
0002-9440(1997)150:4<1189:IACOLE>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYSTIC KIDNEY-DISEASE; P-GLYCOPROTEIN GENE; HOMOZYGOUS DISRUPTION; OVAL CELLS; DIFFERENTIATION; RAT; HEPATOCYTES; EXPRESSION; LINEAGES; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
W.G. Richards et al., "ISOLATION AND CHARACTERIZATION OF LIVER EPITHELIAL-CELL LINES FROM WILD-TYPE AND MUTANT TGN737RPW MICE", The American journal of pathology, 150(4), 1997, pp. 1189-1197

Abstract

The Tg737 gene encodes a tetratricopeptide repeat containing protein that, when disrupted in TgN737Rpw mutant mice, results in pleiotropic phenotypes that include the proliferation of epithelial cells, In the kidney and liver this causes a phenotype that resembles autosomal recessive polycystic kidney disease, In the liver, the affected epithelialcells morphologically, and immunologically resemble oval cells, were we describe the isolation, culture, and characterization of epithelialcell lines derived from the fivers of wild-type, heterozygous, and homozygous TgN737Rpw mice, Essentially homogeneous cell cultures were established and the expression of liver markers was examined by reverse transcriptase polymerase chain reaction and by immunohistochemistry. All of the cell lines reacted to the A6 antibody that was raised against mouse oval cells and expressed markers seen in oval cells, Cells transplanted into the interscapular fat pads of isogenic mice formed welldefined ductular structures, Furthermore, in transfection experiments, we have demonstrated the involvement of Tg737 in cellular proliferation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:50:25