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Titolo:
STRUCTURAL ORGANIZATION OF THE HUMAN VESICULAR MONOAMINE TRANSPORTER TYPE-2 GENE AND PROMOTER ANALYSIS USING THE JELLY FISH GREEN FLUORESCENT PROTEIN AS A REPORTER
Autore:
XU WM; LIU LZ; MOOSLEHNER K; EMSON PC;
Indirizzi:
UNIV LONDON UNIV COLL,RAYNE INST,CRUCIFORM PROJECT,5 UNIV ST LONDON WC1E 6JJ ENGLAND BABRAHAM INST,MRC,MOL NEUROSCI GRP,DEPT NEUROBIOL CAMBRIDGE CB2 4AT ENGLAND
Titolo Testata:
Molecular brain research
fascicolo: 1, volume: 45, anno: 1997,
pagine: 41 - 49
SICI:
0169-328X(1997)45:1<41:SOOTHV>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHROMAFFIN GRANULES; MOLECULAR-CLONING; SOMATOSTATIN GENE; AMINE TRANSPORTER; RESPONSE ELEMENT; EXPRESSION; TRANSCRIPTION; TOXICITY;
Keywords:
GENOMIC STRUCTURE; HUMAN VMAT2; 16 EXON; PROMOTER ANALYSIS; GREEN FLUORESCENT PROTEIN; CYCLIC AMP RESPONSE ELEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
W.M. Xu et al., "STRUCTURAL ORGANIZATION OF THE HUMAN VESICULAR MONOAMINE TRANSPORTER TYPE-2 GENE AND PROMOTER ANALYSIS USING THE JELLY FISH GREEN FLUORESCENT PROTEIN AS A REPORTER", Molecular brain research, 45(1), 1997, pp. 41-49

Abstract

The genomic structure of a human vesicle monoamine transporter, type-2 (hVMAT2) was determined from two overlapping cosmids, phVMAT2-cos1 and phVh4T2-cos2, spanning more than 35 kb. The hVMAT2, open reading Game is encoded by 16 exons, with translation initiation and terminationin exon 2 and exon 16, respectively. Several potential binding sites for transcriptional regulatory factors, including a cAMP response element (CRE) were identified in the 5'-upstream region of the gene. A promoter construct using the jellyfish green fluorescent protein (GFP) asreporter has been made and transfected into the human neuroblastoma cell Line, SHSY-5Y. The cellular expression of the GFP was readily detected by fluorescence microscopy and cells expressing GFP could be sorted using a fluorescence-activated cell sorter (FAGS), allowing the level of GFP expression in transfected SHSY-5Y cells to be quickly and reliably determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:16:33