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Titolo:
AGONIST-ACTIVATED ALPHA-NU-BETA-3 ON PLATELETS AND LYMPHOCYTES BINDS TO THE MATRIX PROTEIN OSTEOPONTIN
Autore:
BENNETT JS; CHAN C; VILAIRE G; MOUSA SA; DEGRADO WF;
Indirizzi:
UNIV PENN,DIV HEMATOL ONCOL,STELLAR CHANCE LABS,SCH MED,DEPT MED,RM 1005,422 CURIE BLVD PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT BIOCHEM & BIOPHYS PHILADELPHIA PA 19104 DUPONT MERCK PHARMACEUT CO WILMINGTON DE 19880
Titolo Testata:
The Journal of biological chemistry
fascicolo: 13, volume: 272, anno: 1997,
pagine: 8137 - 8140
SICI:
0021-9258(1997)272:13<8137:AAOPAL>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ATHEROSCLEROTIC PLAQUES; MURINE MONOCLONAL-ANTIBODY; VITRONECTIN RECEPTOR; INTEGRIN RECEPTOR; GLYCOPROTEIN-IIB; LIGAND-BINDING; SMOOTH-MUSCLE; FIBRINOGEN; EXPRESSION; ALPHA(V)BETA(3);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
J.S. Bennett et al., "AGONIST-ACTIVATED ALPHA-NU-BETA-3 ON PLATELETS AND LYMPHOCYTES BINDS TO THE MATRIX PROTEIN OSTEOPONTIN", The Journal of biological chemistry, 272(13), 1997, pp. 8137-8140

Abstract

The phosphorylated acidic glycoprotein osteopontin is present in the extracellular matrix of atherosclerotic plaques and the wall of injured but not normal arteries. To determine if osteopontin could serve as a substrate for platelet adhesion, we measured the adherence of resting and agonist-stimulated human platelets to immobilized recombinant human osteopontin. Agonist-stimulated but not resting platelets bound toosteopontin by a process that was mediated primarily by alpha v beta 3. alpha v beta 3-mediated adherence occurred at physiologic concentrations of calcium and was inhibited by an alpha v beta 3-selective cyclic peptide. Assays using phorbol myristate acetate-stimulated transfected B lymphocytes expressing both alpha v beta 3 and alpha IIb beta 3 confirmed that activated alpha v beta 3 not activated alpha IIb beta 3was responsible for the cellular adherence we measured. These studiesindicate that alpha v beta 3 can reside on the cell surface in an inactive state and can be converted to a ligand binding conformation by cellular agonists. Moreover, they suggest that platelet adherence to osteopontin mediated by activated alpha v beta 3 could play a role in anchoring platelets to disrupted atherosclerotic plaques and the walls of injured arteries. By inhibiting alpha v beta 3 function, it may be possible to inhibit platelet-mediated vascular occlusion with a minimaleffect on primary hemostasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:32:38