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Titolo:
THE INTRINSIC RADIOSENSITIVITY OF SOME HUMAN TUMOR-CELLS THROUGHOUT THEIR CELL-CYCLES
Autore:
BIADE S; STOBBE CC; CHAPMAN JD;
Indirizzi:
FOX CHASE CANC CTR,DEPT RADIAT ONCOL,TUMOR BIOL & BIOPHYS SECT,7701 BURHOLME AVE PHILADELPHIA PA 19111
Titolo Testata:
Radiation research
fascicolo: 4, volume: 147, anno: 1997,
pagine: 416 - 421
SICI:
0033-7587(1997)147:4<416:TIROSH>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHINESE-HAMSTER CELLS; RADIATION SENSITIVITY; SURVIVAL CURVES; INACTIVATION; FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
S. Biade et al., "THE INTRINSIC RADIOSENSITIVITY OF SOME HUMAN TUMOR-CELLS THROUGHOUT THEIR CELL-CYCLES", Radiation research, 147(4), 1997, pp. 416-421

Abstract

The intrinsic radiosensitivity of tumor cells is most frequently reported for asynchronous populations, although cell cycle variation in radiosensitivity is known to be significant. Linear-quadratic analyses of survival data for asynchronous human tumor cells show wide variations in the cw coefficient with smaller variations in the beta coefficient. HT-29 (colon), OVCAR10 (ovary) and A2780 (ovary) tumor cells with alpha coefficients of 0.03, 0.16 and 0.47 Gy(-1), respectively, and root beta coefficients of 0.23-0.27 Gy(-1) for asynchronous populations were amenable to synchronization by mitotic selection. Selection procedures were optimized for each cell line and produced mitotic populations of >90%, similar to 80% and similar to 65% purity for HT-29, OVCAR10and A2780 cells, respectively. Mitotic cells from each line exhibitedsimilar and maximum radiosensitivities with alpha coefficients of similar to 1.3 Gy(-1) after irradiation with Cs-137 gamma rays and after correction for genome multiplicity. Their relative radiosensitivities observed with asynchronous cells were maintained as they progressed through interphase of the cell cycle. All cells in early G(1) phase exhibited a marked radioresistance relative to their sensitivity in mitosis, and maximum interphase radiosensitivity was observed near the G(1)/S-phase boundary. All cells became increasingly radioresistant as theymoved through S phase, the effect being most pronounced for OVCAR10 cells and least pronounced for A2780 cells. HT-29 cells remained relatively radioresistant in G(2) phase. The different interphase radiosensitivities observed for these cell lines were determined mainly by the single-hit inactivation mechanism. These studies clearly demonstrate the dominant role of single-hit inactivation in determining the intrinsic radiosensitivity of human tumor cells to Cs-137 gamma rays, especially at doses of 2 Gy and less. (C) 1997 by Radiation Research Society.

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Documento generato il 05/07/20 alle ore 22:09:22