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Titolo:
P53 INDUCED BY IONIZING-RADIATION MEDIATES DNA END-JOINTING ACTIVITY,BUT NOT APOPTOSIS OF THYROID-CELLS
Autore:
YANG TT; NAMBA H; HARA T; TAKMURA N; NAGAYAMA Y; FUKATA S; ISHIKAWA N; KUMA K; ITO K; YAMASHITA S;
Indirizzi:
NAGASAKI UNIV,SCH MED,INST ATOM DIS,DEPT CELL PHYSIOL NAGASAKI 852 JAPAN NAGASAKI UNIV,SCH MED,INST ATOM DIS,DEPT CELL PHYSIOL NAGASAKI 852 JAPAN NAGASAKI UNIV,SCH MED,DEPT PHARMACOL NAGASAKI 852 JAPAN ITO HOSP TOKYO 150 JAPAN KUMA HOSP KOBE 650 JAPAN
Titolo Testata:
Oncogene
fascicolo: 13, volume: 14, anno: 1997,
pagine: 1511 - 1519
SICI:
0950-9232(1997)14:13<1511:PIBIMD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; SEQUENCE SIMILARITY; ONCOGENE ACTIVATION; HIGH PREVALENCE; CYCLE CONTROL; EARLY EVENT; GENE; DEATH; BCL-2; EXPRESSION;
Keywords:
IONIZING RADIATION; P53; DNA END-JOINTING; APOPTOSIS; THYROID CELL; THYROID CARCINOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
T.T. Yang et al., "P53 INDUCED BY IONIZING-RADIATION MEDIATES DNA END-JOINTING ACTIVITY,BUT NOT APOPTOSIS OF THYROID-CELLS", Oncogene, 14(13), 1997, pp. 1511-1519

Abstract

To understand the effects of ionizing radiation on thyroid cells, we investigated the role of p53 in mediating apoptosis and in DNA repair following in vivo and in vitro irradiation of thyroid cells, In vitro exposure of human thyroid cells to ionizing radiation of up to 5-8 Gy failed to induce apoptosis in primary cells, The same results were obtained when the thyroid gland was irradiated in the intact rat, To explore the mechanism of failure of the wild-type p53 in inducing apoptosis in thyroid cells, we investigated the expression of apoptosis-related genes, bar, bcl-2 and fas/APO-1 following irradiation or induction of temperature-sensitive p53. The expression of Bar, Bcl-2 and Fas/APO-1 in human primary cultured thyroid cells did not change after irradiation, To further confirm the results, we established a clonal cell line (tsFRO) in which a temperature sensitive p53 (Val138) expression vector was stably transfected to a thyroid carcinoma cell line lacking endogenous p53, Incubation of tsFRO cells at the permissive temperature for three days, however, did not induce apoptosis although G(1) arrestwas noted, Although enhanced expression of the bar mRNA level was observed, the expression of Bar, Bcl-2 and Fas/APO-1 protein did not change by shifting tsFRO cells to permissive temperature as well as irradiated primary cells, Furthermore, DNA end-jointing ability was examinedby transfection of linearized luciferase plasmid into tsFRO cells, Increased luciferase activity occurred when the cells were cultured at the permissive temperature, indicating that the mild-type p53 enhances DNA end-jointing activity, Our results indicate that the wild-type p53does not lead to apoptosis but facilitates DNA end-jointing in thyroid cells, These results may reflect specific responses in thyroid cellsfollowing irradiation.

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Documento generato il 04/04/20 alle ore 15:15:00