Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ACTIVATION OF PROTEIN-KINASE-C AFTER FERTILIZATION IS REQUIRED FOR REMODELING THE MOUSE EGG INTO THE ZYGOTE
Autore:
GALLICANO GI; MCGAUGHEY RW; CAPCO DG;
Indirizzi:
ARIZONA STATE UNIV,DEPT ZOOL,MOL & CELLULAR BIOL PROGRAM ZOOL TEMPE AZ 85287 ARIZONA STATE UNIV,DEPT ZOOL,MOL & CELLULAR BIOL PROGRAM ZOOL TEMPE AZ 85287 UNIV CHICAGO,HOWARD HUGHES MED INST CHICAGO IL 60637
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 46, anno: 1997,
pagine: 587 - 601
SICI:
1040-452X(1997)46:4<587:AOPAFI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACELLULAR FREE CALCIUM; CYTOPLASMIC FREE CALCIUM; CYCLIC ADP-RIBOSE; PHORBOL ESTER; XENOPUS-LAEVIS; MAMMALIAN EGGS; HAMSTER EGGS; INOSITOL 1,4,5-TRISPHOSPHATE; CYTOSKELETAL ELEMENTS; MEIOTIC MATURATION;
Keywords:
FERTILIZATION; POLAR BODY; SIGNAL TRANSDUCTION; CHROMOSOMES; PRONUCLEI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
G.I. Gallicano et al., "ACTIVATION OF PROTEIN-KINASE-C AFTER FERTILIZATION IS REQUIRED FOR REMODELING THE MOUSE EGG INTO THE ZYGOTE", Molecular reproduction and development, 46(4), 1997, pp. 587-601

Abstract

Fertilization of the mammalian egg initiates numerous biochemical andstructural changes which remodel the egg into a single-celled zygote. To date, the most extensively studied phenomenon of fertilization in virtually all species has been the relationship between sperm penetration and the induction of the initial rise in intracellular-free calcium ([Ca2+](i)) concentration within the egg. In contrast, relatively few studies have focused on the biochemical events following this rise in calcium, and even fewer studies have directly linked the biochemicalevents to the structural changes which must ensue for proper development of the embryo. In this study, we exploited recently developed technologies to investigate the action of protein kinase C (PKC), a presumed downstream transducer of the initial vise in [Ca2+](i), during fertilization and artificial activation with calcium ionophore or phorbol 12-myristate 13-acetate (PMA). The newly developed myristoylated PKC pseudosubstrate (myrPKC Psi) was used to specifically inhibit PKC, thereby averting the trauma of injecting the egg with nonmyristoylated PKCPsi. Following fertilization, eggs which were pretreated with myr PKCPsi were not capable of forming a second polar body and pronuclear formation was significantly inhibited. Spatial and temporal localizationof PKC using confocal microscopy to visualize the PKC reporter dye, Rim-1, demonstrated localization of PKC to the lateral aspects of the forming second polar body after fertilization, or after artificial activation with calcium ionophore or PMA. In vivo biochemical analysis of eggs which were fertilized or artificially activated demonstrated thatPKC activity rose at the same time (40 min) as the second polar body formed and then subsided over the next 5 hr post activation. From these data, we conclude that PKC plays an integral vole in directing the transformation from egg to embryo. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:46:42