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Titolo:
EVALUATION OF THE AUTOMATED FLUORESCENT ANALYSIS OF THE H-RAS MINISATELLITE AFTER OPTIMIZED PCR AMPLIFICATION IN COMPARISON WITH A STANDARDIZED SOUTHERN BLOT TECHNIQUE
Autore:
GOSSE S; SAUVAIGO S; DAVER A; LARRA F; SAAVEDRA J; MARCHAND J; BERNARDGALLON D; BIGNON YJ;
Indirizzi:
CTR PAUL PAPIN,LAB RADIOANAL,2 RUE MOLL F-49033 ANGERS FRANCE CIS BIO INT GIF SUR YVETTE FRANCE CEA,CIS BIO INT GRENOBLE FRANCE CTR JEAN PERRIN,INSERM CRI 9402,MOL ONCOL LAB F-63011 CLERMONT FERRAN1 FRANCE
Titolo Testata:
International journal of oncology
fascicolo: 4, volume: 10, anno: 1997,
pagine: 735 - 740
SICI:
1019-6439(1997)10:4<735:EOTAFA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RARE ALLELES; HRAS1 MINISATELLITE; COLORECTAL-CANCER; SUSCEPTIBILITY; POLYMORPHISM; INSTABILITY; CARCINOMAS; MUTATIONS; LOCUS; RISK;
Keywords:
H-RAS MINISATELLITE; AMPLIFICATION; ALLELE SIZE; DNA SEQUENCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
S. Gosse et al., "EVALUATION OF THE AUTOMATED FLUORESCENT ANALYSIS OF THE H-RAS MINISATELLITE AFTER OPTIMIZED PCR AMPLIFICATION IN COMPARISON WITH A STANDARDIZED SOUTHERN BLOT TECHNIQUE", International journal of oncology, 10(4), 1997, pp. 735-740

Abstract

Determination of allele sizes? loss of heterozygosity or genetic instability at minisatellite VNTR loci, are routinely performed by the conventional Southern technique. We have investigated the potential use of automated DNA sequencer for the analysis of the H-ras minisatellite. We report the modifications of amplification parameters and electrophoresis conditions on the sequencer. Seventy-one colorectal carcinomas and the corresponding normal tissues were amplified with fluorescent-labeled primers, analyzed on sequencer, and concurrently controlled by Southern blotting. The results on sequencer showed that a Hydrolink matrix used in non-denaturing conditions and a specific analysis software facilitate a more accurate fragment size calculation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 12:57:00