Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ASSIGNMENT AND ANALYSIS OF FLUORINE NUCLEAR-MAGNETIC-RESONANCE SPECTRA OF 4-FLUOROTRYPTOPHAN MYOGLOBINS AND HEMOGLOBINS
Autore:
PEARSON JG; MONTEZ B; LE HB; OLDFIELD E; CHIEN EYT; SLIGAR SG;
Indirizzi:
UNIV ILLINOIS,DEPT CHEM,600 S MATHEWS AVE URBANA IL 61801 UNIV ILLINOIS,DEPT CHEM URBANA IL 61801 UNIV ILLINOIS,BECKMAN INST,BIOPHYS PROGRAM URBANA IL 61801
Titolo Testata:
Biochemistry
fascicolo: 12, volume: 36, anno: 1997,
pagine: 3590 - 3599
SICI:
0006-2960(1997)36:12<3590:AAAOFN>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSE CHEMOSENSORY RECEPTOR; FLUOROTYROSINE ALKALINE-PHOSPHATASE; D-LACTATE DEHYDROGENASE; SPERM WHALE MYOGLOBIN; ESCHERICHIA-COLI; CHEMICAL-SHIFTS; F-19 NMR; LIGAND-BINDING; DIHYDROFOLATE-REDUCTASE; GROWTH-INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
J.G. Pearson et al., "ASSIGNMENT AND ANALYSIS OF FLUORINE NUCLEAR-MAGNETIC-RESONANCE SPECTRA OF 4-FLUOROTRYPTOPHAN MYOGLOBINS AND HEMOGLOBINS", Biochemistry, 36(12), 1997, pp. 3590-3599

Abstract

We have obtained the 470 MHz F-19 NMR spectra of wild type [4-F]Trp-labeled myoglobins (MbCO, MbO(2), deoxyMb, metMb, and MbCN) and hemoglobins (HbCO, HbO(2), and deoxyHb), as well as those of several mutants (W7F Mb, beta W15F Hb, beta W37S Hb, and beta Y130F Hb, all as the carbonmonoxy adducts), prepared via site-directed mutagenesis. The maximum observed chemical shift range induced by folding is 6.4 ppm. Using amultipole shielding polarizability-local reaction field approach, we have computed the electrostatic field contributions to the fluorine shielding. For residues which do not have F atoms in contact with neighboring groups, we find an similar to 1 ppm mean square deviation in shift from experiment, with the R2-like structure of HbCOA being in very close accord with experiment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:30:21