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Titolo:
HYDROLYTIC ENZYMES AND LECTIN-BINDING ACTIVITY OF BLACK-PIGMENTED ANAEROBIC RODS
Autore:
GRENIER D; LABBE S; MOUTON C; MEYRAND D;
Indirizzi:
UNIV LAVAL,FAC MED DENT,RECH ECOL BUCCALE QUEBEC CITY G1K 7P4 PQ CANADA
Titolo Testata:
Microbiology
, volume: 140, anno: 1994,
parte:, 4
pagine: 873 - 878
SICI:
1350-0872(1994)140:<873:HEALAO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS W50; ORAL BACTERIA; PORPHYROMONAS; ASSAY; GENUS;
Keywords:
PORPHYROMONAS SPP; PREVOTELLA SPP.; BACTEROIDES SPP; LECTIN-BINDING ACTIVITY; HYDROLYTIC ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
D. Grenier et al., "HYDROLYTIC ENZYMES AND LECTIN-BINDING ACTIVITY OF BLACK-PIGMENTED ANAEROBIC RODS", Microbiology, 140, 1994, pp. 873-878

Abstract

Recent taxonomic studies on black-pigmented anaerobic rods, a group of bacteria found on mucosal surfaces of humans and animals, led to thesubdivision of existing species and to the creation of new species. The aim of this study was to characterize all 11 currently recognized species of black-pigmented bacteria (55 strains) for their ability to hydrolyse a variety of natural and synthetic substrates and for their lectin reactivity. Although most of the strains demonstrated some activity against proteinaceous substrates, Porphyromonas gingivalis was theonly species able to hydrolyse type I collagen. Most strains possessed glycylprolyl protease activity, elastase-like activity and phospholipase C activity, whereas trypsin-like activity was restricted to P. gingivalis, Porphyromonas salivosa and Bacteroides macacae. beta-Lactamase activity was demonstrated in five strains belonging to the saccharolytic group. The lectin reactivity of the bacteria was determined by adot-blot procedure using horseradish-peroxidase-conjugated lectins. Three lectins, LOTUS A, RCA-I and ConA, failed to react with any of thebacteria tested. WGA reacted strongly with the cell surface of human biotypes of asaccharolytic black-pigmented bacteria (P. gingivalis, Porphyromonas asaccharolytica and Porphyromonas endodontalis) and Prevotella intermedia. The animal biotype strains of P. gingivalis showed a higher affinity for SBA and PNA than for WGA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:05:46