Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PROBING THE STRUCTURE OF THE HIV-1 REV TRANSACTIVATOR PROTEIN BY FUNCTIONAL-ANALYSIS
Autore:
THOMAS SL; HAUBER J; CASARI G;
Indirizzi:
EUROPEAN MOL BIOL LAB,DEPT BIOCOMP D-69117 HEIDELBERG GERMANY EUROPEAN MOL BIOL LAB,DEPT BIOCOMP D-69117 HEIDELBERG GERMANY SANDOZ GMBH,RES INST,DEPT IMMUNODERMATOL A-1235 VIENNA AUSTRIA
Titolo Testata:
Protein engineering
fascicolo: 2, volume: 10, anno: 1997,
pagine: 103 - 107
SICI:
0269-2139(1997)10:2<103:PTSOTH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS TYPE-1 REV; CAVITY-CREATING MUTATIONS; I REX; DOMAIN; BINDING; MOTIF; MUTANTS; REGION; RNA; IDENTIFICATION;
Keywords:
HIV-1 REV; MUTAGENESIS; PROTEIN STRUCTURE; STRUCTURE DETERMINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
S.L. Thomas et al., "PROBING THE STRUCTURE OF THE HIV-1 REV TRANSACTIVATOR PROTEIN BY FUNCTIONAL-ANALYSIS", Protein engineering, 10(2), 1997, pp. 103-107

Abstract

Human immunodeficiency virus type 1 (HIV-1) encodes a trans-acting regulatory protein, termed Rev, which is critically required for virus replication. Rev is a sequence-specific RNA binding protein which mediates the nuclear export of unspliced and incompletely spliced viral mRNAs encoding the viral structural proteins. While CD and fluorescence measurements have provided several possible structural models of Rev, all attempts employing X-ray crystallography and MMR techniques have sofar failed to provide more accurate data. We present a new approach to validate alternative structural models of the N-terminal region of Rev which contains the nuclear localization/RNA binding domain. Points of contact between structural elements in a protein were determined byintroduction of targeted amino acid substitutions and subsequent scoring of the biological activities. Our data resulted in the suggestion of a new and more refined model of HIV-1 Rev structure which to date has been impossible to obtain by other means.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 22:43:22