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Titolo:
CHARACTERIZATION OF A MITOMYCIN-BINDING DRUG-RESISTANCE MECHANISM FROM THE PRODUCING ORGANISM, STREPTOMYCES-LAVENDULAE
Autore:
SHELDON PJ; JOHNSON DA; AUGUST PR; LIU HW; SHERMAN DH;
Indirizzi:
UNIV MINNESOTA,CTR MICROBIAL PHYSIOL & METAB ENGN,BIOL PROC TECHNOL INST,GORTNER LAB 240 ST PAUL MN 55108 UNIV MINNESOTA,CTR MICROBIAL PHYSIOL & METAB ENGN,BIOL PROC TECHNOL INST,GORTNER LAB 240 ST PAUL MN 55108 UNIV MINNESOTA,DEPT MICROBIOL ST PAUL MN 55108 UNIV MINNESOTA,DEPT CHEM MINNEAPOLIS MN 55455
Titolo Testata:
Journal of bacteriology
fascicolo: 5, volume: 179, anno: 1997,
pagine: 1796 - 1804
SICI:
0021-9193(1997)179:5<1796:COAMDM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; TRANSPORT-SYSTEMS; DNA-SEQUENCES; BLEOMYCIN; GENES; ANTIBIOTICS; CLONING; PROTEIN; RECOGNITION; ALKYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Sheldon et al., "CHARACTERIZATION OF A MITOMYCIN-BINDING DRUG-RESISTANCE MECHANISM FROM THE PRODUCING ORGANISM, STREPTOMYCES-LAVENDULAE", Journal of bacteriology, 179(5), 1997, pp. 1796-1804

Abstract

In an effort to characterize the diversity of mechanisms involved in cellular self-protection against the antitumor antibiotic mitomycin C (MC), DNA fragments from the producing organism (Streptomyces lavendulae) were introduced into Streptomyces lividans and transformants were selected for resistance to the drug. Subcloning of a 4.0-kb BclI fragment revealed the presence of an MC resistance determinant, mrd. Nucleotide sequence analysis identified an open reading frame consisting of 130 amino acids with a predicted molecular weight of 14,364, Transcriptional analysis revealed that mrd is expressed constitutively, with increased transcription in the presence of MC, Expression of mrd in Escherichia coli resulted in the synthesis of a soluble protein with an M(r) of 14,400 that conferred high-level cellular resistance to MC and aseries of structurally related natural products, Purified MRD was shown to function as a drug-binding protein that provides protection against cross-linking of DNA by preventing reductive activation of MC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:00:38