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Titolo:
ISOLATION AND CHARACTERIZATION OF THE HUMAN NUCLEOPHOSMIN B23 (NPM) GENE - IDENTIFICATION OF THE YY1 BINDING-SITE AT THE 5'-ENHANCER REGION/
Autore:
CHAN PK; CHAN FY; MORRIS SW; XIE Z;
Indirizzi:
BAYLOR COLL MED,DEPT PHARMACOL HOUSTON TX 77030 ST JUDE CHILDRENS HOSP,DEPT EXPT ONCOL MEMPHIS TN 38105
Titolo Testata:
Nucleic acids research
fascicolo: 6, volume: 25, anno: 1997,
pagine: 1225 - 1232
SICI:
0305-1048(1997)25:6<1225:IACOTH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOLAR PROTEIN-B23 TRANSLOCATION; MURINE TUMOR-CELLS; HELA-CELLS; TRANSCRIPTIONAL REPRESSION; BCR GENE; B23; LOCALIZATION; NUCLEAR; INDUCTION; NUMATRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
P.K. Chan et al., "ISOLATION AND CHARACTERIZATION OF THE HUMAN NUCLEOPHOSMIN B23 (NPM) GENE - IDENTIFICATION OF THE YY1 BINDING-SITE AT THE 5'-ENHANCER REGION/", Nucleic acids research, 25(6), 1997, pp. 1225-1232

Abstract

NPM (nucleophosmin/BP3) is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal restingcells. Recently, it was found that NPM gene is located at the breakpoints of the t(2:5), 1(3:5) and 1(5:17) chromosome translocation. To understand the human NPM gene's structure and regulation, four genomic clones were isolated from the human chromosome 5 library and their DNA sequences analyzed. The human NPM gene has 12 exons of sizes ranging from 58 to 358 bp. The chromosome breakpoint for 1(2:5) and 1(5:17) translocation is within intron 4 and the breakpoint for 1(3:5) translocation is within intron 6, The initiation site is located 96 bp upstream from the ATG site. A typical TATA box (at -25 nt) and a GC box (at -65nt) were identified in the promoter region. We identified two gel-shift bands (A and B) with DNA fragment E (-741/-250 nt) by EMSA. A DNA footprint was observed at (-371/344 nt) with the nuclear extract. A double stranded DNA with the footprint sequence (-371/-344 nt) competed the formation of gel-shift bands A and B in EMSA suggesting that proteins A and B bind to the footprint region. We confirmed that protein A is transcription factor YY1. These results suggest that YY1 may play a role in NPM gene expression. This is the first report on human NPM gene structure and sequence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 05:00:48