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Titolo:
CELLULAR HANDLING OF UNOCCUPIED AND AGONIST-STIMULATED CHOLECYSTOKININ RECEPTOR DETERMINED BY IMMUNOLOCALIZATION
Autore:
DETOLEDO CF; ROETTGER BF; MORYSWORTMANN C; SCHMIDT WE; MILLER LJ;
Indirizzi:
MAYO CLIN & MAYO FDN,CTR BASIC RES DIGEST DIS,200 1ST ST,SW ROCHESTERMN 55905 MAYO CLIN & MAYO FDN,CTR BASIC RES DIGEST DIS ROCHESTER MN 55905 MAX PLANCK INST EXPT MED,IMMUNOCHEM ABT D-37075 GOTTINGEN GERMANY CHRISTIAN ALBRECHTS UNIV KIEL,LAB MOL GASTROENTEROL,MED KLIN 1 D-24105 KIEL GERMANY
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 3, volume: 35, anno: 1997,
pagine: 488 - 497
SICI:
0193-1857(1997)35:3<488:CHOUAA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC ACINAR-CELLS; SHORT PROBES; PHOSPHORYLATION; PROTEIN; DESENSITIZATION; INTERNALIZATION; DEPHOSPHORYLATION;
Keywords:
RECEPTOR INTERNALIZATION; RECEPTOR PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
C.F. Detoledo et al., "CELLULAR HANDLING OF UNOCCUPIED AND AGONIST-STIMULATED CHOLECYSTOKININ RECEPTOR DETERMINED BY IMMUNOLOCALIZATION", American journal of physiology: Gastrointestinal and liver physiology, 35(3), 1997, pp. 488-497

Abstract

Cellular handling of receptor molecules is an important mechanism forthe regulation of appropriately sensitive hormone-stimulated signaling. Until now, our understanding of the cellular handling of the cholecystokinin (CCK) receptor has been largely limited to following a tagged ligand through the cell. In the present work, we report the application of unique CCK receptor antisera directed toward intracellular domains, which permitted the immunolocalization of this molecule independently of its occupation with ligand. The CCK receptor antisera were also useful in Western blotting and immunoprecipitation of this receptor. Unstimulated CCK receptors remained on the surface of both recombinant stable rat CCK-A receptor-bearing Chinese hamster ovary cell line (CHO-CCKR) cells and native rat pancreatic acinar cells and did not constitutively internalize. Agonist stimulation of the CHO-CCKR cells resulted in the prompt internalization of a subset of surface receptors, representing those that were occupied with ligand. Unoccupied receptorsremained on the surface, uninfluenced by the stimulated signaling pathways. Consistent with this, CCK receptor phosphorylation induced by 12-O-tetradecanoylphorbol-13-acetate treatment did not stimulate receptor internalization. After internalization, we observed substantial receptor recycling to the plasma membrane. These insights provide the first evidence that CCK receptor internalization occurs as a direct result of an induced conformational change and presumed bimolecular interaction, rather than as an effect of a signaling event.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 18:53:47