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Titolo:
BASIC FIBROBLAST GROWTH FACTOR-INDUCED ANGIOGENIC PHENOTYPE IN MOUSE ENDOTHELIUM - A STUDY OF AORTIC AND MICROVASCULAR ENDOTHELIAL-CELL LINES
Autore:
BASTAKI M; NELLI EE; DELLERA P; RUSNATI M; MOLINARITOSATTI MP; PAROLINI S; AUERBACH R; RUCO LP; POSSATI L; PRESTA M;
Indirizzi:
UNIV BRESCIA,DEPT BIOMED SCI & BIOTECH,UNIT GEN PATHOL & IMMUNOL,VIA VALSABBINA 19 I-25123 BRESCIA ITALY UNIV BRESCIA,DEPT BIOMED SCI & BIOTECH,UNIT GEN PATHOL & IMMUNOL I-25123 BRESCIA ITALY UNIV BRESCIA,DEPT BIOMED SCI & BIOTECH,UNIT HISTOL I-25123 BRESCIA ITALY UNIV WISCONSIN,DEPT ZOOL MADISON NJ 00000 UNIV ROMA LA SAPIENZA,DEPT EXPT MED & PATHOL,IMMUNOPATHOL UNIT ROME ITALY UNIV ANCONA,INST BIOMED SCI ANCONA ITALY
Titolo Testata:
Arteriosclerosis, thrombosis, and vascular biology
fascicolo: 3, volume: 17, anno: 1997,
pagine: 454 - 464
SICI:
1079-5642(1997)17:3<454:BFGFAP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; ANTIFIBRINOLYTIC DRUGS; HEPARAN-SULFATE; PHORBOL ESTER; RECEPTOR; EXPRESSION; INVITRO; TUMORS; VASCULARIZATION; INDUCTION;
Keywords:
ANGIOGENESIS; ENDOTHELIUM; MOUSE; BASIC FIBROBLAST GROWTH FACTOR; PLASMINOGEN ACTIVATORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
M. Bastaki et al., "BASIC FIBROBLAST GROWTH FACTOR-INDUCED ANGIOGENIC PHENOTYPE IN MOUSE ENDOTHELIUM - A STUDY OF AORTIC AND MICROVASCULAR ENDOTHELIAL-CELL LINES", Arteriosclerosis, thrombosis, and vascular biology, 17(3), 1997, pp. 454-464

Abstract

The mouse is the most commonly used species for in vivo studies on angiogenesis related to tumor development. Yet, to the best of our knowledge, very few reports on the in vitro interaction of the angiogenic basic fibroblast growth factor (bFGF) with mouse endothelial cells are available. Three mouse endothelial cell lines originated from aorta (MAECs), brain capillaries (MBECs), and heart capillaries (MHECs) were characterized for endothelial phenotypic markers, in vivo tumorigenic activity, and the capacity to respond in vitro to bFGF. These cells express angiotensin-converting enzyme, acetylated LDL receptor, constitutive endothelial nitric oxide synthase, and vascular cell adhesion molecule-1 and bind Griffonia simplicifolia-I lectin. When injected subcutaneously in nude mice, MAECs induced the appearance of slow-growing vascular lesions reminiscent of epithelioid hemangioendothelioma, whereas MBEC xenografts grew rapidly, showing Kaposi's sarcoma-like morphological features. No lesions were induced by injection of MHECs. MAECs, MBECs, and MHECs expressed both low-affinity heparan sulfate bFGF-binding sites and high-affinity tyrosine kinase receptors (FGFRs) on theirsurfaces. In particular, MAECs expressed FGFR-2/bek mRNA, whereas microvascular MBECs and MHECs expressed FGFR-1/flg mRNA. According, bFGF induced a mitogenic response and the phosphorylation of extracellular signal-regulated kinase-2 in all the cell lines. In contrast, upregulation of urokinase-type plasminogen activator expression was observed in bFGF-treated microvascular MBECs and MHECs but not in MAECs. Also, bFGF-treated MBECs and MHECs but not MAECs invaded a three-dimensional fibrin gel and formed hollow, capillary-like structures. The relevanceof the modifications of the fibrinolytic balance of mouse microvascular endothelium in bFGF-induced angiogenesis was validated in vivo by agelatin-sponge assay in which the plasmin inhibitors tranexamic acid and epsilon-aminocaproic acid given to mice in the drinking water inhibited neovascularization induced by the growth factor. In conclusion, differences in response to bFGF exist between large-vessel MAECs and microvascular MBECs and MHECs. Both in vitro and in vivo data point to a role of the profibrinolytic phenotype induced by bFGF in microvascular endothelial cells during mouse angiogenesis. Our observations make these endothelial cell lines suitable for further studies on mouse endothelium during angiogenesis and in angioproliferative diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 10:37:15