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Titolo:
INTERLEUKIN-10-INDUCED AND CORTICOSTEROID-INDUCED REDUCTION IN TYPE-IPROCOLLAGEN IN A HUMAN EX-VIVO SCAR CULTURE
Autore:
WANGOO A; LABAN C; COOK HT; GLENVILLE B; SHAW RJ;
Indirizzi:
UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,SCH MED,DEPT RESP MED,NORFOLK PL LONDON W2 1PG ENGLAND UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,SCH MED,DEPT RESP MED LONDON W2 1PG ENGLAND UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,SCH MED,DEPT HISTOPATHOL LONDON W2 1PG ENGLAND ST MARYS HOSP,DEPT CARDIOTHORAC SURG LONDON ENGLAND
Titolo Testata:
International journal of experimental pathology
fascicolo: 1, volume: 78, anno: 1997,
pagine: 33 - 41
SICI:
0959-9673(1997)78:1<33:IACRIT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; PULMONARY FIBROSIS; TOPICAL CORTICOSTEROIDS; ALVEOLAR MACROPHAGES; COLLAGEN-SYNTHESIS; MESSENGER-RNA; FIBROBLASTS; EXPRESSION; INTERFERON; BIOSYNTHESIS;
Keywords:
FIBROSIS; INTERLEUKIN-10; TYPE I PROCOLLAGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
A. Wangoo et al., "INTERLEUKIN-10-INDUCED AND CORTICOSTEROID-INDUCED REDUCTION IN TYPE-IPROCOLLAGEN IN A HUMAN EX-VIVO SCAR CULTURE", International journal of experimental pathology, 78(1), 1997, pp. 33-41

Abstract

Fibroblasts act as the effector cells of the fibrotic response via production of collagen. In an attempt to understand the regulation of fibroblasts from areas of active human tissue fibrosis, we have developed an ex vivo model in which biopsies of scars from patients 6 weeks post thoracotomy were cultured. This model has been used to investigate whether interleukin-10 (IL-10) and triamcinolone acetonide modulate the expression of type I procollagen mRNA and protein. In situ hybridization and a quantitative competitive RT-PCR were used to measure type procollagen mRNA. Type I procollagen protein was evaluated by immunochemistry. Viability of biopsies in culture using H-3-uridine incorporation into RNA was observed to be > 80% for at least 96 hours. Following addition of either IL-10 or triamcinolone acetonide there was a modestbut significant decrease (P < 0.05) in type I procollagen mRNA expression. Similarly, each agent added individually to biopsies reduced theproportion of cells staining positively for type I procollagen when compared to biopsies treated with medium alone (P < 0.05). These results extend in vitro data that IL-10 and corticosteroids down-regulate collagen synthesis in skin fibroblast cell lines and suggest that this ex vivo model may offer a closer approximation to the post-operative scarring process when testing new therapeutic agents for reducing an over-exuberent fibrotic response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:54:56