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Titolo:
SINGLE AMINO-ACID RESIDUE AS A FUNCTIONAL DETERMINANT OF ROD AND CONEVISUAL PIGMENTS
Autore:
IMAI H; KOJIMA D; OURA T; TACHIBANAKI S; TERAKITA A; SHICHIDA Y;
Indirizzi:
KYOTO UNIV,FAC SCI,DEPT BIOPHYS KYOTO 60601 JAPAN KYOTO UNIV,FAC SCI,DEPT BIOPHYS KYOTO 60601 JAPAN
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 6, volume: 94, anno: 1997,
pagine: 2322 - 2326
SICI:
0027-8424(1997)94:6<2322:SARAAF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PUTATIVE TRANSMEMBRANE SEGMENTS; SCHIFF-BASE COUNTERION; BOVINE RHODOPSIN; METARHODOPSIN-II; MOLECULAR-PROPERTIES; CHICKEN GREEN; COLOR-VISION; EXPRESSION; MEMBRANE; SEQUENCE;
Keywords:
RHODOPSIN; G PROTEIN; RETINA; VISION; MOLECULAR EVOLUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
H. Imai et al., "SINGLE AMINO-ACID RESIDUE AS A FUNCTIONAL DETERMINANT OF ROD AND CONEVISUAL PIGMENTS", Proceedings of the National Academy of Sciences of the United Statesof America, 94(6), 1997, pp. 2322-2326

Abstract

The visual transduction processes in rod and cone photoreceptor cellsbegin with photon absorption by the different types of visual pigments, Cone visual pigments exhibit faster regeneration from 11-cis-retinal and opsin and faster decay of physiologically active intermediate (meta II) than does the rod visual pigment, rhodopsin, as expected, due to the functional difference between rod and cone photoreceptor cells,To identify the amino acid residue(s) responsible for the difference in molecular properties between rod and cone visual pigments, we selected three amino acid positions (64, 122, and 150), where cone visual pigments have amino acid residues electrically different from those of rhodopsin, and prepared mutants of rhodopsin and thicken green-sensitive cone visual pigment, The results showed that the replacement of Glu-122 of rhodopsin by the residue containing green- or red-sensitive cone pigment converted rhodopsin's rates of regeneration and meta II decay into those of the respective cone pigments, whereas the introduction of Glu-122 into green-sensitive cone visual pigment changed the rates of these processes into rates similar to those of rhodopsin, Furthermore, exchange of the residue at position 122 between rhodopsin and chicken green-sensitive cone pigment interchanges their efficiencies in activating retinal G protein transducin, Thus, the amino acid residue at position 122 is a functional determinant of rod and cone visual pigments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:39:08