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Titolo:
REMOVAL OF A CRYPTIC INTRON AND SUBCELLULAR-LOCALIZATION OF GREEN FLUORESCENT PROTEIN ARE REQUIRED TO MARK TRANSGENIC ARABIDOPSIS PLANTS BRIGHTLY
Autore:
HASELOFF J; SIEMERING KR; PRASHER DC; HODGE S;
Indirizzi:
MRC,MOL BIOL LAB,DIV CELL BIOL,HILLS RD CAMBRIDGE CB2 2QH ENGLAND US ANIM & PLANT HLTH INSPECT SERV,USDA,OTIS PLANT PROTECT CTR OTIS MA02542
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 6, volume: 94, anno: 1997,
pagine: 2122 - 2127
SICI:
0027-8424(1997)94:6<2122:ROACIA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; SELECTION; MUTATIONS; SEQUENCES; REPORTER; ANIMALS; CELLS;
Keywords:
PLANT TRANSFORMATION; CONFOCAL MICROSCOPY; CRYPTIC SPLICING; GENETIC MARKER; ENDOPLASMIC RETICULUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J. Haseloff et al., "REMOVAL OF A CRYPTIC INTRON AND SUBCELLULAR-LOCALIZATION OF GREEN FLUORESCENT PROTEIN ARE REQUIRED TO MARK TRANSGENIC ARABIDOPSIS PLANTS BRIGHTLY", Proceedings of the National Academy of Sciences of the United Statesof America, 94(6), 1997, pp. 2122-2127

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is finding wide use as a genetic marker that can be directly visualized in the living cells of many heterologous organisms, We have sought to express GFP in the model plant Arabidopsis thaliana but have found that proper expression of GFP is curtailed due to aberrant mRNA processing, An 84-nt cryptic intron is efficiently recognized and excisedfrom transcripts of the GFP coding sequence. The cryptic intron contains sequences similar to those required for recognition of normal plant introns, We have modified the codon usage of the gfp gene to mutate the intron and to restore proper expression in Arabidopsis. GFP is mainly localized within the nucleoplasm and cytoplasm of transformed Arabidopsis cells and can give rise to high levels of fluorescence, but itproved difficult to efficiently regenerate transgenic plants from such highly fluorescent cells, However, when GFP is targeted to the endoplasmic reticulum, transformed cells regenerate routinely to give highly fluorescent plants, These modified forms of the gfp gene are useful for directly monitoring gene expression and protein localization and dynamics at high resolution, and as a simply scored genetic marker in living plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 04:58:30