Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MODIFICATION OF ANNEXIN-II EXPRESSION IN PC12 CELL-LINES DOES NOT AFFECT CA2-DEPENDENT EXOCYTOSIS()
Autore:
GRAHAM ME; GERKE V; BURGOYNE RD;
Indirizzi:
UNIV LIVERPOOL,PHYSIOL LAB LIVERPOOL L69 3BX MERSEYSIDE ENGLAND UNIV MUNSTER,CLIN RES GRP ENDOTHELIAL CELL BIOL D-48149 MUNSTER GERMANY
Titolo Testata:
Molecular biology of the cell
fascicolo: 3, volume: 8, anno: 1997,
pagine: 431 - 442
SICI:
1059-1524(1997)8:3<431:MOAEIP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-DEPENDENT EXOCYTOSIS; ADRENAL CHROMAFFIN CELLS; CALPACTIN-I; CA2+-ACTIVATED SECRETION; REGULATED EXOCYTOSIS; ELECTRON-MICROSCOPY; CYTOSOLIC PROTEINS; BINDING PROTEINS; PLASMA-MEMBRANE; P36 SUBSTRATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
M.E. Graham et al., "MODIFICATION OF ANNEXIN-II EXPRESSION IN PC12 CELL-LINES DOES NOT AFFECT CA2-DEPENDENT EXOCYTOSIS()", Molecular biology of the cell, 8(3), 1997, pp. 431-442

Abstract

The Ca2+/phospholipd/cytoskeletal-binding protein annexin II has beenproposed to play an important role in Ca2+-dependent exocytosis; however, the evidence for this role is inconclusive. More direct evidence obtained by manipulating annexin II levels in cells is still required. We have attempted to do this by generating stably transfected PC12 cell lines expressing proteins which elevate or lower functional annexinII levels and using these cell lines to investigate Ca2+-dependent exocytosis. Three cell lines were generated: one expressing an annexin II mutant which aggregates annexin II in at least a proportion of the cells, thereby removing functional protein from the cell; a mixed clonal cell line constitutively overexpressing human annexin II; and a clonal cell line capable of overexpressing annexin II in the presence of sodium butyrate. After digitonin permeabilization, Ca2+-dependent dopamine release from these cell lines was compared with that from control nontransfected cells, and, in addition, release was compared in induced to uninduced cells. There were no significant differences in Ca2+-dependent exocytosis between any of the transfected cell lines before orafter induction and the control cells. In addition, nontransfected PC12 cells treated with nerve growth factor, which elevates annexin II levels severalfold, failed to increase Ca2+-dependent exocytosis after digitonin permeabilization, compared with control cells. We conclude that annexin II is not an important regulator of Ca2+-dependent exocytosis in PC12 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 03:57:47