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Titolo:
DIFFERENCES OF MICROVASCULAR ENDOTHELIUM IN THE BOVINE CORPUS-LUTEUM OF PREGNANCY AND THE CORPUS-LUTEUM OF THE ESTROUS-CYCLE
Autore:
PLENDL J; NEUMULLER C; SINOWATZ F;
Indirizzi:
UNIV MUNICH,INST VET ANAT,VET STR 13 D-80539 MUNICH GERMANY
Titolo Testata:
Biology of the cell
fascicolo: 3, volume: 87, anno: 1996,
pagine: 179 - 188
SICI:
0248-4900(1996)87:3<179:DOMEIT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; NECROSIS-FACTOR-ALPHA; CORPORA-LUTEA; BLOOD-VESSELS; GRIFFONIA-SIMPLICIFOLIA; HEPATIC LIPASE; IN-VITRO; CELLS; HETEROGENEITY; LOCALIZATION;
Keywords:
ENDOTHELIUM; PREGNANCY; CORPUS LUTEUM; LECTINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
J. Plendl et al., "DIFFERENCES OF MICROVASCULAR ENDOTHELIUM IN THE BOVINE CORPUS-LUTEUM OF PREGNANCY AND THE CORPUS-LUTEUM OF THE ESTROUS-CYCLE", Biology of the cell, 87(3), 1996, pp. 179-188

Abstract

The purpose of the present study was to investigate potential modulations of endothelial cells of the bovine corpus luteum (CL) during pregnancy. Luteal endothelia of pregnant and non-pregnant cows were isolated and purity of cultures was verified by flow cytometric quantification of three independent endothelial markers (von Willebrand factor, angiotensin converting enzyme, Bandeiraea simplicifolia agglutinin I ligands). Different cellular parameters including light and electron microscopical investigation of morphology and growth characteristics as well as quantification of cellular lectin binding sites were compared. Extensive heterogeneity between luteal endothelial cells in pregnant and non-pregnant animals could be demonstrated, reflected in functional attributes like angiogenic activity, ultrastructural characteristics and the quantitative expression of cellular carbohydrates. Two different morphological types of cells ('cobblestone growth pattern' and 'arcuate growth pattern') were isolated from the CL of pregnancy as well asfrom the cyclic CL. Spontaneous angiogenic activities, including cellular migration in band-like structures and formation of ring-like structures, were observed in endothelial cells isolated from the CL of pregnant cows exclusively. This strongly suggests that microvascular luteal endothelium of pregnant animals, in contrast to the one of non-pregnant animals, is able to produce quantitatively and/or qualitatively specific angiogenesis factor(s). Heterogeneity between luteal endothelial cells in the pregnant and non-pregnant animal could also be demonstrated by quantification of lectin (Bandeiraea simplicifolia agglutininI, concanavalin A, Dolichos biflorus agglutinin, Ulex europaeus agglutinin I, wheat germ agglutinin) binding sites: quantitative expressionof specific endothelial cell surface carbohydrates could be correlated to the status of pregnancy, thus emphasizing the actual need of quantification of lectin binding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:29:25