Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIRECT RECOGNITION OF RAT MHC ANTIGENS ON RAT ANTIGEN-PRESENTING CELLS BY MOUSE CD4(-CELLS AND ESTABLISHMENT OF T-CELL CLONES EXHIBITING A DIRECT RECOGNITION PATHWAY() AND CD8(+) T)
Autore:
HIROTA T; HIROSE H; IWATA H; KANETAKE K; MURAKAWA S; SASAKI E; TAKAGI H; BANDO M; HAMAOKA T; FUJIWARA H;
Indirizzi:
GIFU UNIV,SCH MED,DEPT SURG 1,40 TUKASA MACH GIFU 500 JAPAN OSAKA UNIV,SCH MED,BIOMED RES CTR OSAKA 553 JAPAN
Titolo Testata:
Transplantation
fascicolo: 5, volume: 63, anno: 1997,
pagine: 705 - 710
SICI:
0041-1337(1997)63:5<705:DRORMA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-II GENES; HELPER-CELLS; COSTIMULATORY SIGNAL; GRAFT-REJECTION; MINIATURE SWINE; CD4+; CD28; ACTIVATION; RESPONSES; PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
T. Hirota et al., "DIRECT RECOGNITION OF RAT MHC ANTIGENS ON RAT ANTIGEN-PRESENTING CELLS BY MOUSE CD4(-CELLS AND ESTABLISHMENT OF T-CELL CLONES EXHIBITING A DIRECT RECOGNITION PATHWAY() AND CD8(+) T)", Transplantation, 63(5), 1997, pp. 705-710

Abstract

Alloantigens are recognized by T cells either through a direct pathway, which involves recognition of alloantigens expressed on allogeneic antigen-presenting cells (APC), or through an indirect pathway, which involves recognition of processed alloantigens presented by self APC. We investigated whether rat xenoantigens are also recognized by direct(xenogeneic APC-restricted) and/or indirect (self APC-restricted) pathways. C57BL/6 (B6) mouse anti-F344 or WKAH rat mixed lymphocyte reactions (MLRs) were partially inhibited by addition of either anti-mouse CD4 or CD8 monoclonal antibody (mAb) and almost completely blocked in the presence of both mAbs. These xenogeneic MLRs were almost completely inhibited by simultaneous depletion of both self and xeno APCs and only partially suppressed by the elimination of either type of APC, indicating that freshly prepared splenic mouse T cells can recognize rat xenoantigens through both direct and indirect pathways, Anti-F334 T cell lines were generated from B6 anti-F344 MLR cultures, and four CD4(+) and four CD8(+) T cell clones were isolated from these parental lines, The parental lines and those derived T cell clones were tested for their ability to proliferate depending on the presence of F344 APC. Proliferation of CDS clones by stimulation with F344 APC was inhibited by the addition of anti-rat class I MHC mAb but not of anti-class II MHC mAbs. Conversely, proliferation of CD4 clones was reduced by addition of anti-class II MHC mAbs. Thus, these results indicate that xeno (rat)-reactive mouse T cells recognize xenoantigens via both indirect (self APC-restricted) and direct (xeno APC-restricted) pathways and thatboth CD4 and CD8 subsets of T cells participate in a direct pathway of xenoantigen recognition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:10:31