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Titolo:
METALLOPROTEINASE ACTIVITY IN MAMMARY ART ERIES AND SAPHENOUS VEINS -MODIFICATION BY DOXYCYCLINE
Autore:
GREZ R; GUARDA E; ACEVEDO C; IRARRAZAVAL MJ;
Indirizzi:
PONTIFICIA UNIV CATOLICA CHILE,FAC MED & CIENCIAS BIOL,DEPT ENFERMEDADES CARDIOVASC SANTIAGO CHILE PONTIFICIA UNIV CATOLICA CHILE,FAC MED & CIENCIAS BIOL,DEPT ENFERMEDADES CARDIOVASC SANTIAGO CHILE
Titolo Testata:
Revista Medica de Chile
fascicolo: 1, volume: 125, anno: 1997,
pagine: 7 - 14
SICI:
0034-9887(1997)125:1<7:MAIMAE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
SPA
Soggetto:
CREVICULAR FLUID COLLAGENASE; MATRIX METALLOPROTEINASES; TETRACYCLINE-INHIBITION; ATHEROSCLEROTIC PLAQUES; BYPASS CONDUITS; GINGIVAL; LOCALIZATION; THERAPY; CELLS;
Keywords:
METALLOPROTEINS; ARTERIES; ARTERIOSCLEROSIS; DOXYCYCLINE; HEART DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
R. Grez et al., "METALLOPROTEINASE ACTIVITY IN MAMMARY ART ERIES AND SAPHENOUS VEINS -MODIFICATION BY DOXYCYCLINE", Revista Medica de Chile, 125(1), 1997, pp. 7-14

Abstract

Background: Alterations in the synthesis and degradation of extracellular matrix occur during atherogenesis. Metalloproteinases, whose activity may be inhibited with doxycicline in other tissues, play an important role in this process. Aims: 1. To characterize metalloproteinasesactivities in internal mammary artery and saphenous vein, and 2. To assess the effect of doxycicline in the activity of metalloproteinases of these vessels and of cultured smooth muscle cells. Methods: Segments of internal mammary arteries and saphenous veins and cultured smoothmuscle cells were incubated with and without doxycicline. Metalloproteinases activity was assessed by zymography and Western Blot. Results:Activity of metalloproteinase-9 in saphenous veins was 217% less thanin internal mammary arteries. In these vessels doxycicline decreased metalloproteinase-9 activity by 207% and metalloproteinase-2 by 290%. Western Blot analysis showed that doxycicline also inhibited metalloproteinase-1 expression. In cultured smooth muscle cells, the median inhibitory concentration of doxycicline for metalloproteinase-2 was 138 mu M (r(2)=0.82). Conclusions: Internal mammary arteries and saphenous veins have different metalloproteinase activities, that are inhibited by doxycicline.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 23:43:13