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Titolo:
A SEMIAUTOMATED SOLID-PHASE EXTRACTION AND RADIORECEPTOR ASSAY FOR THE ANALYSIS OF SCOPOLAMINE IN URINE AND PLASMA
Autore:
BOSMAN IJ; DOUMA WR; ENSING K; DEZEEUW RA;
Indirizzi:
FAC PHARM UTRECHT,DEPT PHARMACEUT ANAL,SORBONNELAAN 16 NL-3584 CA UTRECHT NETHERLANDS UNIV GRONINGEN,GRONINGEN INST DRUG STUDIES,CTR PHARM,DEPT ANAL CHEM &TECHNOL NL-9713 AV GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT PULMONOL NL-9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
European journal of pharmaceutical sciences
fascicolo: 6, volume: 5, anno: 1997,
pagine: 315 - 325
SICI:
0928-0987(1997)5:6<315:ASSEAR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Keywords:
RADIORECEPTOR ASSAY; SOLID-PHASE EXTRACTION; TRANSDERMAL ADMINISTRATION; SCOPOLAMINE PLASMA; URINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
I.J. Bosman et al., "A SEMIAUTOMATED SOLID-PHASE EXTRACTION AND RADIORECEPTOR ASSAY FOR THE ANALYSIS OF SCOPOLAMINE IN URINE AND PLASMA", European journal of pharmaceutical sciences, 5(6), 1997, pp. 315-325

Abstract

In a double-blind placebo-controlled cross-over study, we evaluated the therapeutic efficacy of transdermal scopolamine in ten patients with reversible airways obstruction. They received a patch (Scopoderm(R) TTS) behind the ear for three days and samples of blood and urine weretaken. A highly sensitive method was developed and validated to measure the low levels of free and total scopolamine in urine and plasma. The procedure consisted of a semi-automated solid-phase extraction followed by the analysis using radioreceptor assays. The mean plasma concentrations, taken every third patch day, of free and total scopolamine were 43.6 pg/ml and 229.0 pg/ml, respectively. In 24-h urine, collected every second patch day, 6.3 mu g of free scopolamine and 83.4 mu g total scopolamine was excreted. This means that 70% of the delivered dose (120 mu g in 24 h) is excreted in urine. For urine samples, the limit of detection (LOD) of the assay is 550 pg/ml and the limit of quantitation (LOQ) is 610 pg/ml. For plasma samples, the LOD is 16 pg/ml and the LOQ is 38 pg/ml. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:51:12