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Titolo:
REGULATION OF ZAP-70 INTRACELLULAR-LOCALIZATION - VISUALIZATION WITH THE GREEN FLUORESCENT PROTEIN
Autore:
SLOANLANCASTER J; ZHANG WG; PRESLEY J; WILLIAMS BL; ABRAHAM RT; LIPPINCOTTSCHWARTZ J; SAMELSON LE;
Indirizzi:
NICHHD,CELL BIOL & METAB BRANCH,SECT LYMPHOCYTE SIGNALING,NIH,BLDG 18T,RM 101 BETHESDA MD 20892 NICHHD,CELL BIOL & METAB BRANCH,SECT LYMPHOCYTE SIGNALING,NIH BETHESDA MD 20892 NICHHD,CELL BIOL & METAB BRANCH,UNIT ORGANELLE BIOL,NIH BETHESDA MD 20892 MAYO CLIN & MAYO FDN,DEPT IMMUNOL ROCHESTER MN 55905
Titolo Testata:
The Journal of experimental medicine
fascicolo: 10, volume: 186, anno: 1997,
pagine: 1713 - 1724
SICI:
0022-1007(1997)186:10<1713:ROZI-V>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL ANTIGEN RECEPTOR; SEVERE COMBINED IMMUNODEFICIENCY; TANDEM SH2 DOMAINS; TYROSINE KINASES; PHOSPHOLIPASE C-GAMMA-1; MONOCLONAL-ANTIBODIES; SIGNAL-TRANSDUCTION; ACTIVATION MOTIFS; T-CELLS; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
J. Sloanlancaster et al., "REGULATION OF ZAP-70 INTRACELLULAR-LOCALIZATION - VISUALIZATION WITH THE GREEN FLUORESCENT PROTEIN", The Journal of experimental medicine, 186(10), 1997, pp. 1713-1724

Abstract

To investigate the cellular dynamics of ZAP-70, we have studied the distribution and regulation of its intracellular location using a ZAP-70 green fluorescent protein chimera. Initial experiments in epithelialcells indicated that ZAP-70 is diffusely located throughout the quiescent cell, and accumulates at the: plasma membrane upon cellular activation, a phenotype enhanced hy the coexpression of Lck and the initiation of: ZAP-70 kinase activity. Subsequent studies in T cells confirmed this phenotype. Intriguingly, a large amount of ZAP-70, both chimeric and endogenous, resides in the nucleus of quiescent and activated cells. Nuclear ZAP-70 becomes tyrosine phosphorylated upon stimulation via the T cell receptor, indicating that it may have an important biologic function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:56:19