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Titolo:
THE SITE AND TIME OF EXPRESSION OF MIF IN FROG DEVELOPMENT
Autore:
FUKUZAWA T; OKUMOTO H; NISHIOKA M;
Indirizzi:
KEIO UNIV,DEPT BIOL,KOHOKU KU,HIYOSHI 4-1-1 YOKOHAMA KANAGAWA 223 JAPAN HIROSHIMA UNIV,LAB AMPHIBIAN BIOL HIROSHIMA JAPAN
Titolo Testata:
Pigment cell research
fascicolo: 6, volume: 10, anno: 1997,
pagine: 401 - 409
SICI:
0893-5785(1997)10:6<401:TSATOE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOCYTE-STIMULATING-HORMONE; MOUSE AGOUTI GENE; CELL-MIGRATION; PIGMENT-CELLS; COAT COLOR; CREST; RECEPTOR; PROTEIN; MELANIZATION; MUTATIONS;
Keywords:
PIGMENT PATTERN; MELANIZATION; PIGMENT CELL; MELANOPHORE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
T. Fukuzawa et al., "THE SITE AND TIME OF EXPRESSION OF MIF IN FROG DEVELOPMENT", Pigment cell research, 10(6), 1997, pp. 401-409

Abstract

A ventrally localized melanization-inhibiting factor (MIF) has been suggested to play a role in the expression of dorsal-ventral pigment patterns in amphibia. Here we investigate the onset and localization of MIF appearance in frog development. The expression of MIF was analyzedin the wild-type and gray-eyed mutant (g/g) of Rana japonica by immunoblotting and immunohistochemistry using an anti-MIF neutralizing monoclonal antibody. Western blot analysis revealed that the anti-MIF antibody recognized similar to 51 kDa and similar to 58 kDa bands. The 51 kDa band first appeared at the external gill stage, while 58 kDa band was additionally detected at the hindlimb bud stage. With the use of immunohistochemistry, it was found that the anti-MIF antibody stained the whole epidermis of the embryos at the external gill stage; however,the staining was stronger in lateral and ventral epidermis than in dorsal. Staining with the anti-MIF antibody was observed only in the outer epidermis of the ventral skin, but not in the dorsal skin during and after metamorphosis. The spatial expression of MIF in the wild-type was the same as that in the gray-eyed mutant. The same immunohistochemical result was obtained in the adults of R. nigromaculata. These results suggest that MIF is involved in the formation of the dorsal-ventral pigment pattern.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:07:32